EFFECT OF LONG-TERM PHYSICAL INACTIVITY ON OXlDATIVE STRESS AND TOTAL SERUM ANTIOXIDANT STATUS

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671408
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: JIKEI UNIVERSITY SCHOOL OF MEDICINE
• Principal Investigator: KOYAMA Teruyuki JlKEI UNIVERSITY SCHOOL OF MEDICINE, RESEARCH ASSISTANT, 医学部, 助手 (30234917)
• Co-Investigator(Kenkyū-buntansha): HIDEKI Yamauchi JIKEI UNIVERSITY SCHOOL OF MEDICINE, LECTURER, 医学部, 講師 (60220224)
• Project Period (FY): 2001 – 2002
• Keywords: PHYSICAL INACTIVITY / HINDLIMBS SUSPENDED / OXIDATIVE STRES / RAT / VITAMIN E / SERUM ANTIOXIDANT STATUS / LIPID PEROXID / AGING

Research Abstract

We investigated changes in oxidative stress and total serum antioxidant status (TSAS) after long-term physicalinactivity (PI) with or without vitamin E supplement. Adult (7 months old) and senescent (25 months old) Fischer 344-female rats were assigned into following three groups; control, PI and vitamin E treated PI group. Animals of Pf and vitamin E treated PI groups were kept withtheir hindlimbs suspended (HS) condition for 3 weeks. Vitamin E (RRR-α-tocopherol) was injected every other day (50 mg/kg I.p.) throughout the HS period increasing 6 days prior to HS.
Thiobarbituric acid reactive substance (TBARS) in serumand skeletal muscle increased in senescent age group. PI decreased serum TBARS in both adult and senescent agegroups. TBARS in skeletal muscle tended to decrease in adult age and did not change in senescent age group. Vitamin E supplement increased vitamin E level in serum and skeletal muscle and decreased TBARS. TSAS decreased after PI in senescent age group. These results indicate resistance to oxidative stress appears tb decrease. TSAS decrease by PI was not ameliorated by vitamin E supplement.
In summary, it is thought that oxidative stress decreases by the lowering oxygen consumption during PI. The return to daily-activities from PI is likely to increase acute oxygen consumptioik and production in reactive oxygen species. Therefore, antioxidant vitamin supplement during PI may be beneficial for protecting the organ or tissue damages from oxidative stress, especially in senescent-age that is susceptible to oxidative damage. Since TSAS is not improved by only vitamin E supplement, well-balanced supplement of antioxidants will be recommended to enhance defensive potentials.