2002 Fiscal Year Final Research Report Summary
Analysis of the antigen presentation machinery in malignant glioma cells
| Project/Area Number |
13671427
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | University of Yamanashi (Faculty of Medicine) |
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Principal Investigator |
SATOH Eiji University of Yamanashi, Faculty of Medicine, Research Associate, 医学部, 助手 (10235319)
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| Co-Investigator(Kenkyū-buntansha) |
NAGANUMA Hirofumi University of Yamanashi, Faculty of medicine, associate professor, 医学部, 助教授 (90189142)
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| Project Period (FY) |
2001 – 2002
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| Keywords | malignant glioma / transporter associated with antigen processing / interferon-β |
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| Research Abstract |
Transporter associated with antigen processing (TAP) is necessary for peptide transport and antigen presentation. We investigated a expression of TAP1 in malignant glioma cells and the effect of IFN-γ, IFN-β on the expression of TAP1. We also investigated the polymorphism of TAP1 promoter. We analyzed the sequence of TAP1 promoter in eight malignant glioma cells. Single nucleotide polymorphism occurred in six of eight malignant glioma cells. This polymorphism is a G-T substitution 446 bp upstream of the translation start of TAP1. The expression of TAP1 in all malignant glioma cells was very low. IFN-γ and IFN-β increased the protein and mRNA expression of TAP1. The polymorphism of TAP1 promoter did not have functional effects on the induction of TAP1 expression by IFN-γ and IFN-β.
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