Research about expression of MT5-MMP and invasiveness in human glioma cells

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671476
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Hyogo College of Medicine
• Principal Investigator: NAKANO Atsuhisa Hyogo College of Medicine, Faculty of Medicine, Research Associate, 医学部, 助手 (70258151)
• Co-Investigator(Kenkyū-buntansha): IKEMOTO Hideyasu Hyogo College of Medicine, Faculty of Medicine, Research Associate, 医学部, 助手 (30278824) ; ARITA Norio Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (80159508)
• Project Period (FY): 2001 – 2002
• Keywords: metalloproteinase / invasion / glioma / 浸潤

Research Abstract

A cDNA encoding a new member of the membrane-type (MT) matrix metalloproteinase (MMP) family has been identified and cloned from a human brain cDNA library. This novel human MMP has been called MT5-MMP because it represents the fifth member of the MT-MMP subfamily of MMPs. Northern blot analysis demonstrated that MT5-MMP is predominantly expressed in brain, kidney, pancreas, and lung. In addition, MT5-MMP transcripts were detected at high levels compared to normal brain tissue in a series of brain tumors, including astrocytomas and glioblastomas. The catalytic domain of MT5-MMP exhibits a potent proteolytic activity against progelatinase A, leading to the generation of the Mr 62,000 active form of this enzyme. These data suggest that MT5-MMP may contribute to the activation of progelatinase A in tumor tissues, in which it is overexpressed, thereby facilitating tumor progression.

Research Products

• [Publications] Velasco G, Nakano A, et al.: "Human MT6-matrix metalloproteinase identification, progelatinase A activation, and expression in brain tumors"Cancer Res.. 60. 877-882 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mori K, Nakano A, Arita N, et al.: "Brain-specific angiogenesis inhibitor 1 (BAI1) is expressed in human cerebral neuronal cells"Neurosci Res.. 43. 69-74 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kanemura Y, Nakano A, Arita N, et al.: "Musashil, an evolutionarily conserved neural RNA-binding protein, is versatile marker of human glioma cells in determining their cellular origin, malignancy, and proliferative activity"Differentiation. 68. 141-152 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Velasco G, Cal S, Merlos-Suarez A, Ferrando AA, Alvarez S, Nakano A, Arribas J, Lopez-Otin C: "Human MT6-matrix metalloproteinase : Identification, progelatinase A activation, and expression in brain tumors"Cancer Res. 60(4). 877-882 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kanemura Y, Mori K, Sakakibara S, Fujikawa H, Hayashi H, Nakano A, Matsumoto T, Tamura K, Imai T, Ohnishi T, Fushiki S, Nakamura Y, Yamasaki M, Okano H, Arita N: "Musashi1, an evolutionarily conserved neural RNA-binding protein, is a versatile marker of human glioma cells in determining their cellular origin, malignancy, and proliferative activity"Differentiation. 68(2-3). 141-152 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mori K, Kanemura Y, Fujikawa H, Nakano A, Ikemoto H, Ozaki I, Matsumoto T, Tamura K, Yokota M, Arita N: "Brain-specific angiogenesis inhibitor 1 (BAl1) is expressed in human cerebral neuronal cells"Neurosci Res. 43(1). 69-74 (2002)
  • Description: 「研究成果報告書概要(欧文)」より