2003 Fiscal Year Final Research Report Summary
Effects of anesthesia related drugs on neuronal apoptosis : alterations of enzymatic activities elicited by intracellular calcium elevation.
| Project/Area Number |
13671562
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Gunma University |
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Principal Investigator |
SAITO Sigeru GUNMA UNIVERSITY, School of Medicine, Associate professor, 医学部, 助教授 (40251110)
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| Project Period (FY) |
2001 – 2003
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| Keywords | local anesthetics / nerve growth cone / growth cone collapse / neurotoxicity / neurotrophic factor / intracellular calcium concentration |
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| Research Abstract |
Local anesthetics impair morphology of neurons and disturb physiological nerve conduction. Especially, lidocaine and tetracaine are most toxic among the commercially available local anesthetics. Apoptosis is one of the basic mechanism of the neurotoxity, however, recent publications described that the toxicity could not be thoroughly explained by apoptosis. Growth cones at the tip of growing neurons have been demonstrated as labile to the neurotoxicity of local anesthetics. Several neurotrophic factors promote the recovery of neurons that are exposed to local anesthetics. In the present study, we have demonstrated that mepivacaine, bupivacaine and ropivacaine have similar neurotoxity to growth cones and growing neurites, and that neurotrophic factors, NT-3, BDNF, GDNF, have potential to promote neuronal survival and regeneration following the injury by local anesthetics. Intracellular calcium ion elevation was observed after the exposure to tetracaine beginning from the tip of growing neurite. The way of elevation was not spiky but slowly elevating, and implied impairment of membrane integrity.
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Research Products
(12 results)
