2002 Fiscal Year Final Research Report Summary
The effects of N-acetylcysteine on the mitochondrial function after cerebral ischemia
| Project/Area Number |
13671581
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
TAKEDA Yoshimasa University Hospital, Assistant Professor, 医学部附属病院, 助手 (30294466)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Kiyoshi Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (40108171)
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| Project Period (FY) |
2001 – 2002
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| Keywords | mitochondria / free radical / reperfusion injury |
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| Research Abstract |
Although it is known that development of lipid peroxidation following ischemia occurs predominantly in vulnerable regions, temporal profiles of antioxidants following ischemia have not been regionally elucidated. After reperfusion periods of 0, 3, 24 and 72 hours following 20 minutes of four-vessel occlusion (n = 6 in each group), the concentration of total glutathione (GSH) and the activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-Px) were assayed in the hippocampus, parietal cortex, striatum, thalamus and brain stem. The levels of all antioxidants were unchanged in all regions without reperfusion. However, the concentration of total GSH significantly decreased in the hippocampus at 3 hours after the onset of reperfusion and showed a maximum decrease in the hippocampus (68% of the sham-control level), parietal cortex (78% of the sham-control level) and striatum (76% of the sham-control level) after 24 hours of reperfusion. After 72 hours of reperfusion, these regions and the thalamus showed restoration and an increase in the total GSH concentration, respectively. The activities of SOD, GSH-Px and catalase were stable during the reperfusion period, but the hippocampus showed significant increases in these enzyme activities and the parietal cortex and striatum showed significant increases in SOD activities at 72 hours after the onset of reperfusion. These results indicate that endogenous antioxidants take 72 hours for restoration in vulnerable regions following 20 minutes of four-vessel occlusion in rats.
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