2004 Fiscal Year Final Research Report Summary
Mechanisms of neuronal cell death and treatments after spinal cord ischemia
| Project/Area Number |
13671606
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Nara Medical University |
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Principal Investigator |
KAWAGUCHI Masahiko Nara Medical University, Dept.of Anesthesiology, Assistant Professor, 医学部, 講師 (60275328)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Satoki Nara Medical University, Dept.of Anesthesiology, Assistant Professor, 医学部, 講師 (50295789)
FURUYA Hitoshi Nara Medical University, Dept.of Anesthesiology, Professor, 医学部, 教授 (70183598)
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| Project Period (FY) |
2001 – 2004
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| Keywords | Spinal cord ischemia / cell death / rat |
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| Research Abstract |
For the assessments of spinal cord injury following the spinal cord ischemia, gray matter injury has been mainly focused as a target for therapy, because of traditional view that white matter is less vulnerable to ischemic injury compared with the gray matter. However, recent evidence suggested an importance of white matter injury as well as gray matter injury following spinal cord ischemia. We quantitatively assessed the gray and white matter injury following spinal cord ischemia in rats. Spinal cord ischemia(SCI) was induced with intra-aortic balloon catheter and hypotension. Twenty-four hours after reperfusion, hind limb motor function was assessed using Basso, Beattie, Bresnahan(BBB) scale scoring. The white matter damage was assessed on the basis of the extent of vacuolation and amyloid precursor protein(APP) immunoreactivity. The results indicated that both gray and white matters were injured following spinal cord ischemia in rats and the degree of white mater injury was well correlated with the severity of gray matter injury and hind-limb motor function. Although low-dose lidocaine did not affect the degree of gray and white matter injury, intra-thecal administration of delta opioid agonist SNC80 attenuated both gray and white matter injury following spinal cord ischemia in rats.
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