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2003 Fiscal Year Final Research Report Summary

Sulfotransferase in polycystic kidney

Research Project

Project/Area Number 13671666
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionOsaka City University

Principal Investigator

SUGIMURA Kazunobu  Osaka City University, Graduate School of Medicine, Associate Professor, 大学院・医学研究科, 助教授 (90187659)

Project Period (FY) 2001 – 2003
KeywordsPolycystic Kidney / DPT / Differential display / sulfotransferase / SULT1C2 / recombinant protein
Research Abstract

Background. The pathogenesis of polycystic kidney disease (PKD) remains unclear in spite of the identification of the genes responsible for hereditary PKDs. In this study, we investigated the alteration of gene expressions in an acquired PKD model induced by 2-amino-4,5-diphenylthiazole (DPT) using the differential display method.
Methods. Kidney mRNA from a Sprague-Dawley rat fed with 1% DPT for 4 days and from a control rat was compared by RT-PCR differential display method. Differentially expressed bands were re-amplified and subcloned. Using these subclones as probes, the changes in gene expressions were confirmed by Northern blot analysis. Subsequently, mouse kidney cDNA library was screened.
Results. The isolated 1.5-kb cDNA contained an open reading frame encoding 296 amino acids which shared 94.3% identity with rat SULT1C2 sulfotransferase, and was considered to be its mouse orthologue (GenBank Accession No. AY005469). Mouse SULT1C2 mRNA was abundant in the kidney and stomach among normal mouse tissues. The expression of SULT1C2 mRNA was decreased in the rat kidney after DPT feeding but not in the stomach. Mouse SULT 102 was expressed successfully using pET plasmid vector and F.coil The recombinant 34kDa protein was capable of catalyzing the sulfation of p-nitrophenol at Km of 3.1 mM, by utilizing 3'-phosphoadenosine 5'-phosphosulfate (PAPS) as the sulfate donor.
Conclusions. Although the physiological substrate and function of SULT1C2 have yet to be elucidated, its down-regulation could be involved in the cystic changes of tubules by decreasing the sulfation of the tubular basement membrane components.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] K.Sugimura, et al.: "Decreased sulfotransferase SULT1C2 gene expression in DPT-induced polycystic kidney"Kidney International. 62. 757-762 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazunobu Sugimura, et al.: "Decreased sulfotransferase SULT1C2 gene Expression in DPT-induced poplycystic kidney"Kidney International. 62. 757-762 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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