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2002 Fiscal Year Final Research Report Summary

Study of effect of a carbohydrate chain gene on the characteristics of ovarian cancer cells

Research Project

Project/Area Number 13671755
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionSt. Marianna University School of Medicine

Principal Investigator

KIGUCHI Kazushige  St. Marianna University School of Medicine, Department of Obstetrics and Gynecology, Assistant professor, 医学部, 助教授 (60101911)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Norio  St. Marianna University School of Medicine, Department of Obstetrics and Gynecology, Resident, 医学部, 助手 (10267619)
IWATA Masanori  St. Marianna University School of Medicine, Department of Obstetrics and Gynecology, Resident, 医学部, 助手 (80257401)
Project Period (FY) 2001 – 2002
KeywordsOvarian cancer / carbohydrate chain gene / fucosyltransferase / type I carbohydrate chain
Research Abstract

In this study, we investigated how a carbohydrate chain gene affects the cell characteristics of ovarian cancer and its prognosis. That is, how the properties of the cancer cells were affected by the change In carbohydrate chains of the cells induced by introduction of the gene of fucosyltransferase that is an enzyme modifying non-reducing end of carbohydrate chains of cell membrane was examined. The role of sugar chains at that time was also analyzed. It is known that H gene is participating not only in exhibiting ABO blood types but also in functioning such as cell adhesion. Hence, clarification of biochemical changes in carbohydrate chains of cell membrane induced by introduction of H gene that is engaged in synthesis of carbohydrate chains was attempted, and the following results were obtained.
Results
1. Cells to which human H gene was introduced were smaller and showed denser cell-to-cell adhesion compared with the control cells.
2. Cellular sialic acid content in the gene-introduced cells was low, indicating antagonistic activity between fucosylation and *lation.
3. Since lacto-series type I carbohydrate chains were detected in only the gene-introduced cells, fucosylation seemed to be induced by the introduced gene.
4. Susceptibility to 5-Fu was examined. Owing to introduction of fucosyltransferase, the membrane permeability to hydrophobic molecules rose, and the susceptibility of the cells to anticancer drugs increased.
These results suggested that modification of surface carbohydrate chains of ovarian cancer cells may be applied to the treatment of ovarian cancer for improvement of the prognosis.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Isamu ISHIWATA: "Human Papillomavirus Types 56 and 59 in Novel Cervical Cancer Cell Lines"HUMAN CELL. 13・1. 1-2 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Isamu ISHIWATA: "New Approach for the Establishment of Mouse early Embryonic stem Cell and Induction of their Differentiation"HUMAN CELL. 14・4. 1-2 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kyoko Takehara: "Expression of Glycolipids Bearing Lewis Phenotypes in Tissues and Cultured Cells of Human Gvnecological Cancers"Jpn. J. Cancer Res. 93. 1129-1137 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sojiro SATO: "Establishment and Characterization of a Cell-line Originated from Human Mucinous Cvstadenocarcinoma of the Ovarv"HUMAN CELL. 15・3. 171-177 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Isamu ISHIKAWA: "Human Papillomavirus Types 56 and 59 in Novel Cervical Cancer Cell Lines"HUMAN CELL. 13(1). 1-2 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isamu ISHIKAWA: "New Approach for the Establishment of Mouse early Embryonic stem Cell and Induction of their Differentiation"HUMAN CELL. 14(4). 1-2 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kyoko Takehara: "Expression of Glycolipids Bearing Lewis Phenotypes in Tissues and Cultured Cells of Human Gynecological Cancers"Jpn. J. Cancer Res. 93. 1129-1137 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sojiro SATO: "Establishment and Characterization of a Cell-line Originated from Human Mucinous Cystadenocarcinoma of the Ovary"HUMAN CELL. 15(3). 171-177 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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