2002 Fiscal Year Final Research Report Summary
Autologous graft of the tissue engineered ear
| Project/Area Number |
13671888
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kinki University |
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Principal Investigator |
HIGASHI Tsuyoshi Kinki University, School of Medicine, lecturer, 医学部附属病院, 講師 (70268431)
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| Co-Investigator(Kenkyū-buntansha) |
ISOGAI Noritaka Kinki University, School of Medicine, Assistant Professor, 医学部附属病院, 助教授 (90203067)
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| Project Period (FY) |
2001 – 2002
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| Keywords | polymer / chondrocyte |
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| Research Abstract |
In order to regenerate cartilage with specific three dimensional morphologic shape of auricle, we made a cell-polymer construct which was composed of biodegradanble copolymer and cultured bovine chondrocytes. The polymer which possesses specific character of slow degradation and improved physical properties was subsequently seeded with chondrocytes and then implanted into subcutaneous space of the nude mice to regenerate the auricle-shaped cartilage in vivo (experiment 1). In 11 week specimens after implantation, neo-cartilage was induced from the cell-polymer constructs that retained original three dimensional shape of the auricle cartilage. In experiment 2, in order to extend the maintenance duration of the three dimensional shape, we applied periosteum onto this cell-polymer constructs and investigated how periosteum would affect the re-differentiation of the chondrocytes and three dimensional shape of neo-cartilage. Result revealed mat the newly formed cartilage became thick with the helical rim and anti-helix contour being less prominent. It was therefore concluded that it is possible to induce and maintain a natural auricle-shaped cartilage by the additional inclusion of the periosteum onto cell-polymer constructs. The search for the appropriate site and method for the periosteum attachment to the cell-polymer constructs in the autologous model using large animal is needed as a next step.
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Research Products
(1 results)
