2002 Fiscal Year Final Research Report Summary
Induction of maxillofacial oragns from undifferentiated primitive ectodermal cells by activin A
| Project/Area Number |
13671921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
FURUE Miho Kanagawa Dental College, School of Dentsitry, Lecturer, 歯学部, 講師 (80257310)
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| Co-Investigator(Kenkyū-buntansha) |
ASASHIMA Makoto University of Tokyo, Graduate School of Arts and Sciences, Professor, 総合文化研究科, 教授 (00090564)
HATA Rhyichiro Kanagawa Dental College, School of Dentsitry, Professor, 歯学部, 教授 (10014276)
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| Project Period (FY) |
2001 – 2002
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| Keywords | mazilofacial / activin / jaw / caltilage / differentiation / chondrocyte |
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| Research Abstract |
Activin A has potent mesoderm-inducing activity and induces various mesodermal tissues in vitro from the isolated presumptive Xenopus ectoderm. By using a sandwich culture method, activin-treated ectoderm can function as both a head and trunk-tail organizser, depending on the concentration of activin A. By using activin A and undifferentiated presumptive ectoderm, it is theoretically possible to reproduce embryonic induction. We tested this hypothesis by studying the induction of maxillofacial cartilage tissue by using the sandwich-culture method. In the sandwiched explants, the mesenchymal cell condensation expressed type II collagen and cartilage homeoprotein-1 mRNA, and subsequently, cartilage were induced as they are in vivo. goosecoid (gsc) mRNA was prominenthy expressed in the cartilage in the explants. Xenopus distal-less 4 (X-dll4) mRNA was expressed throughout the explants. In Xenopus embryos, gsc expression is restricted to the cartilage of the lower jaw, and X-dll4 is widely expressed in the ventral head region, including craniofacial cartilage. These finding suggest that the craniofacial cartilage, especially lower jaw cartilage, was induced in the activin-treated sandwiched explants. In addition, a normal developmental pattern was recapitulated at the histological and genetic level. This work also suggests that the craniofacial cartilage-induction pathway is downstream of activin A. This study presents a model system suitable for the in vitro analysis of craniofacial cartilage induction in vertebrates.
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Research Products
(10 results)
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[Publications] Furue, M., Zhang, Y., Okamoto, T., Hata, R., Asashima, M.: "Activin A induces expression of rat sel-1l mRNA, a negative regulator of Notch signaling, in rat salivary gland-derived epithelial cells"Biochemical and Biophysical Research Communications. 282. 745-749 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Furue,M., Myoishi,Y., Fukui,Y., Ariizumi,T., Okamoto,T., Asashima,M.: "Activin A induces craniofacial cartilage from undifferentiated Xenopus ectoderm in vitro"Proceedings of the National Academy of Sciences of the United States of America. 99. 15474-15479 (2002)
Description
「研究成果報告書概要(欧文)」より
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