2003 Fiscal Year Final Research Report Summary
Possibility of GVT effects on oral malignant nepolasms
| Project/Area Number |
13671977
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
OHNO Jun Fukuoka Dental College, Lecturer, 歯学部, 講師 (10152208)
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| Project Period (FY) |
2001 – 2003
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| Keywords | GVHD / GVT / cytokines / CTL / immunohistochemistry / malignant melanoma / metastasis / tumor immunology |
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| Research Abstract |
The results obtained were divided into three parts ; 1) effects of MCP-1 producing macrophages on the development of local GVH reactions in the oral mucosa, 2) GVT effects on oral melanoma in mice, and 3) biosynthesis of sialic acid on the terminal portion of glycoconjugates can affect effects of GVT against oral melanoma in mice. First, in local GVH reaction of the oral mucosa, macrophages infiltrate into the upper region of lamina propria after an expression of ICAM-1 in epithelial cells. We suggest that MCP-1, produced by macrophages, may be responsible for the chemotactic factor of CTL into surface epithelia of the oral mucosa. Second, GVT effects may depend on degree of systemic GVH reaction. In advanced stages of systemic GVHD, CTL infiltrated directly into nests of melanoma cells (B16 ; C57BL/6-origin), whereas less infiltration of CTL was noted in the early phase. We suggested that GVT effects were induced by immunological pathway of acute oral mucosal GVHD. However, CTL reaction against tumor cells were not specific, because they also attacked oral mucosa, ispecially epithelial cells. Third, the cell surface glycoconjugates of melanoma cells injected were modified during their development in the oral mucosa. We examined histochemically an attachment of sialic acid on the terminal portion of glycoconjugates in melanoma cells. Binding of sialic acid on the cell surface may responsible for susceptibleness of metastasis into submandibular lymph nodes and for difficulties of GVT effects.
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