The analysis of tissue regeneration of pulp tissue using the derivation on stem cell differentiation

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671994
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tohoku University
• Principal Investigator: SHOJI Shigeru Tohoku University, Hospital, Lecturer, 歯学部附属病院, 講師 (10142986)
• Co-Investigator(Kenkyū-buntansha): NEMOTO Eiji Tohoku University, Graduate School of Dentistry, Assistant, 大学院・歯学研究科, 助手 (40292221) ; YAMAKI Keiko Tohoku University, Graduate School of Dentistry, Assistant, 大学院・歯学研究科, 助手 (90182419)
• Project Period (FY): 2001 – 2003
• Keywords: Stem cell / differentiation / pulp / regeneration / immunohistochemistry

Research Abstract

The fragility of pulp-less tooth has been well known. Besides the loss of time and money through the root canal treatment for a patient and a dentist. To solve this problem, we thought that it was worthwhile for pulp treatment to derive the partial pulp tissue using the derivation on stem cell differentiation.
Recently the development of research on cell facial antigen of embryonic stem cell (ES cell) has been remarkable. Especially the Stage Specific Embryonic Antigen-1 (SSEA-1) stained the cell facial antigen of ES cell, Embryonal Carcinoma cell (EC cell) and Embryonic Germ cell (EG cell) on mouse, and on human cell SSEA-1 stains just only EG cell. But the Stage Specific Embryonic Antigen-4 (SSEA-4) stains all human ECIES and EG cell, and this SSEA-4 could not be observed in mouse cells.
We stained these antigens on human and mouse pulp tissue cells. On human cells we could observe all ES,EC and ES antigens. Then this 2003 we tried to stain another antigen (Tumor Rejection Antigen-1-60: TRA-1-60) and TRA-1-81. we could stain these antigen on human pulp cells. But we could not bather the human pulp tissue under the definite condition. We must advance the immunohistochemical research under the definite condition using mouse cells.
Moreover we need the establishment to distinguish the difference among the ES,EC and EG cells. If we can establish these difference, we will get the pulp tissue regeneration using the derivation on stem cell differentiation.