2003 Fiscal Year Final Research Report Summary
Microbicidal efficacy ozonated water against Candida albicans adhered to acrylic denture plate
| Project/Area Number |
13672047
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
ARITA Masahiro Kyushu Dental College, School of Dentistry, Assistant Professor, 歯学部, 講師 (50184289)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIHARA Tatsuji Kyushu Dental College, School of Dentistry, Professor, 歯学部, 教授 (80192251)
FUKUIZMI Takaki Kyushu Dental College, School of Dentistry, Assistant Professor, 歯学部, 講師 (50275442)
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| Project Period (FY) |
2001 – 2003
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| Keywords | ozonated water / Candida albicans / denture plaque / denture cleaning / disinfection |
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| Research Abstract |
<Objectives> Ozone is known to act as a strong antimicrobial agent against bacteria, fungi, and viruses. We examined the effect of ozonated water against. Candida albicans on acrylic denture plate. <Methods> The heat-cured acrylic resins were cultured with C. albicans. Following washing with sterile saline and immersion in ozonated water (4 mg/L) for 60 minutes, numbers of the attached C.albicans was counted. In some experiments, the test samples were treated with ozonated water in combination with ultrasonication. <Results> After exposure to flowing ozonated water (2 or 4 mg/L) for 1 minute, viable C.albicans cells were nearly nonexistent. The combination of ozonated water and ultrasonication had a strong effect on the viability of C.albicans adhered to the acrylic resin plates. There were no significant differences in antimicrobial activity against C.albicans between plates immersed in ozonated water with ultrasonication and those treated with commercially available denture cleaners. In addition, electron microscopic analysis revealed that small amounts of C.albicans were remained on the plate, after exposure to flowing ozonated water or immersion in ozonated water with ultrasonication. <Conclusion> Our results suggested that application of ozonated water may be useful for reducing the number of C.albicans on the denture plates.
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