2002 Fiscal Year Final Research Report Summary
Effect of transmembrane proteoglycans in human periodontal ligament fibroblasts
| Project/Area Number |
13672154
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
SHIMAZU Atsushi Graduate School of Biomedical Sciences, Research Assistant, 大学院・医歯薬学総合研究科, 助手 (10274094)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Yukio Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (10112062)
MORISHITA Masayuki Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (90166405)
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| Project Period (FY) |
2001 – 2002
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| Keywords | periodontal ligament / basic fibroblast growth factor / matrix metalloproteinase (MMP)-3 |
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| Research Abstract |
Basic fibroblast growth factor (bFGF, FGF-2) is one of the potent mitogens for periodontal ligament (PDL) cells. However, the role of bFGF on the matrix metalloproteinase (MMP) -3 expression in PDL cells is unknown. In this study, the effect of bFGF on MMP-3 expression in PDL cells and the mechanism of this process were examined.
Human PDL cells were exposed to bFGF at various concentrations (0.01 - 10 ng/ml) in monolayer cultures. bFGF increased [3H]-thymidine incorporation and suppressed proteoglycan synthesis concentration-dependently. However, similar concentration ranges of bFGF increased the release of the cell-associated proteoglycans into the medium. Furthermore, bFGF increased MMP-3 mRNA levels concentration-dependently as examined by reverse transcription-polymerase chain reaction. Induction of MMP-3 after the stimulation with bFGF was observed as early as 12 h with maximal at 24 h. Thereafter the MMP-3 mRNA level gradually decreased until 72 h. Cycloheximide blocked the induction of MMP-3 by bFGF, indicating the requirement of de novo protein synthesis for this stimulation. Furthermore, MMP-3 expression induced by bFGF was abrogated by U0126, a specific inhibitor of MEK1/2 and ERK1/2 in mitogen-activated protein (MAP) kinase pathway, not by PD98059, a specific inhibitor of MEK1. In addition, bFGF up-regulated the phosphorylated ERK1/2 in 5 min with the maximal at 20 min as examined by Western blotting, and U0126 inhibited the ERK1/2 phosphorylation induced by bFGF.
These findings suggest that bFGF induces MMP-3 expression in PDL cells through the activation of the MEK2 in MAP kinase pathway. bFGF stimulation on MMP-3 synthesis may be involved in the control of the cell-associated proteoglycans in PDL cells during periodontal regeneration and degradation.
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Research Products
(10 results)
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[Publications] Okazaki, M., Yamazaki, Y., Yoshida, Y., Shimazu, A., Nokihara, K., Hamada, Y., Takahashi, J. and Matsuura, N.: "A new concept of CO3 apatite-collagen composites with adhesion motif as biomaterials"Dentistry in Japan. 37. 95-100 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tsutsumi, S., Shimazu, A., Miyazaki, K., Pan, H., Koike, C., Yoshida, E., Takagishi, K. and Kato, Y.: "Retention of Multi-lineage differentiation potential of mesenchymal cells during proliferation in response to FGF"Biochem.Biophys.Res.Commun.. 288. 413-419 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamasaki, Y., Yoshida, Y., Okazaki, M., Shimazu, A., Uchida, T., Kudo, T., Akagawa, Y., Hamada, Y. and Matsuura, N.: "Synthesis of functionally graded MgCO3 apatite accelerating osteoblast adhesion"J.Biomed.Mater.Res.. 62. 99-105 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Suardita, K., Fujimoto, K., Oda, R., Shimazu, A., Miyazaki, K., Kawamoto, T. and Kato, Y.: "Effects of overexpression of membrane-bound transferrin-like protein (MTf) on chondrogenic differentiation in vitro"J.Biol.Chem.. 277. 48579-48586 (2002)
Description
「研究成果報告書概要(欧文)」より
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