2003 Fiscal Year Final Research Report Summary
Effect of-mechanicalstress on osteoblast and osteoclast like cell
| Project/Area Number |
13672160
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKAO Noriko Nagasaki University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 助手 (20333578)
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| Co-Investigator(Kenkyū-buntansha) |
TATAMIYA Mitsuhito Nagasaki University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 助手 (20346919)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Centrifugal force / Pulsing electromagnetic fields / Human osteoblast-like cell / Osteoclast / Intracellular response / Prostaglandin / Making to lime / Resorption of the bone |
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| Research Abstract |
The mechanical stress, which were both the gravity and the electromagnetic field, stimulated the ALPase activity in a dose. dependent manner. The results suggested that the mechanical stress induced the differentiation of the human osteoblast-like cell. Furthermore, the pulse electromagnetic held load inhibited ALPase activity in the cell culture before the cell confluent. It. was clear that the pulse electromagnetic held load effected the human osteoblast-like cell in the proliferation the period. F6-1, 25(OH)2D3 and 1-25(OH)2D3 also effected the stimulation of the human osteoblast-like cell proliferation by the gravity and the electromagnetic field. In general, the increase of ALPase activity and the production of BGP, which was non-collagen substrate protein, were related each other. But, the different production of BMP in the gravity and in the pulse electromagnetic field was recognized in this study. However, the gravity, which was the centrifugal force, did not effect both the RANKL-and TNF-α-induced osteoclastogenesis. These results suggested that the mechanical was useful for bone formation because it induced the osteoblast-formation but not the osteoclastogenesis.
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[Publications] Kitaura, H., Nagata, N., Fujimura, Y., Hotokezaka, H., Yoshida, N., Nakayama, K.: "Effect of IL-12 on TNF-a-mediated osteoclast formation in bone marrow cells : apoptosis mediated by Fas/Fas ligand interactionl"Journal of Immunology. 169(9). 4732-4738 (2002)
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「研究成果報告書概要(欧文)」より
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