2002 Fiscal Year Final Research Report Summary
Novel mechanism of action of monoclonal nonspecific suppressor factor (MNSF)
| Project/Area Number |
13680715
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Shimane Medical University |
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Principal Investigator |
NAKAMURA Morihiko Shimane Medical University Medical Associate professor, 医学部, 助教授 (20155865)
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| Co-Investigator(Kenkyū-buntansha) |
TANIGAWA Yoshinori Shimane Medical University Medical Professor, 医学部, 教授 (60084860)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Ubiquitin / Suppressor / Signal transduction |
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| Research Abstract |
Monoclonal nonspecific suppressor factor (MNSF), a lymphokine produced by murine T cell hybridoma, possesses pleiotrophic antigen-nonspecific suppressive function. We have shown that 70-kDa MNSF comprises an 8-kDa ubiquitin-like polypeptide (Ubi-L) and 62-kDa TCRα-like molecule. Ubi-L binds specifically to its 82-kDa receptor protein on target cells. In the current study, We have further characterized the biochemical nature of the TCRa-like molecule. The 62-kDa protein was separated into two species of 46-kDa and 16-kDa on reverse-phase HPLC. Anti-TCRα monoclonal antibody recognized the 46-kDa, but not 16-kDa protein. Anti-TCRβ monoclonal antibody failed to recognize these proteins. Ubi-L conjugated to the 46-kDa protein, whereas Ubi-L lacking its C-termiai Gly-Gly did not. Although Ubi-L was labile both to heating at 56℃ and to acidification to pH 4, Ubi-L46-kDa protein complex was unaffected by these treatments. In addition, the 46-kDa protein elongated the Ubi-L-induced protein tyrosine phosphorylations in concanavalin A-activated murine T helper type 2 clone, D10 cells. One of the four tryptic peptide sequences derived from the 46-kDa protein was in alignment with a related sequence found in the Ja regjon of the TCRa, including the highly conserved motif F-G-X-G-T-X-L.
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Research Products
(6 results)
