2002 Fiscal Year Final Research Report Summary
Modification of host genes in the infected cells with parvovirus
| Project/Area Number |
13680907
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YAGAMI Kenichi Univ. of Tsukuba, Basic Medicine, Prof., 基礎医学系, 教授 (40166476)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Fumihiro Univ. of Tsukuba, Basic Medicine, A. Prof., 基礎医学系, 助教授 (90226481)
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| Project Period (FY) |
2001 – 2002
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| Keywords | parvovirus / rat / thymic lymphoma cell / subtraction / CNTFR_α |
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| Research Abstract |
Rat parvovirus (RPV) propagates in rat thymic lymphoma C58(NT)R cell line, and induces apoptosis. Resistant cells survived after infection reveal different phenotypes such as resistance to apoptosis and tummor suppression compared to parent cell clone. To elucidate the moleculer mechanism of this phenomenon, we tried to identify the genes which express differently between the resistant cells and the parental cell. From the results of representational difference analysis (RDA), 6 known genes and 2 unknown genes were determined. Cathepsin E, thymocyte ARPP and seladin-1 were down-expressed in the parental C58 (NT) cells, and ciliary neurotrophic factor receptor alpha (CNTFR_α), protein associating with small stress protein 1, and envelop gene of Molony murine leukemia virus were enhanced expression in the resistant cell clone, by PCR and Northern blot analysis. These results suggest that enhanced expresson of CNTFR_α contribute to induce resistance to apoptosis in the resistant cell clone.
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