2002 Fiscal Year Final Research Report Summary
Establishment of the novel evaluation method for measurement of pain n animals using wavelet analysis
| Project/Area Number |
13680915
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tohoku Pharmaceutical University |
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Principal Investigator |
ANDO Ryuichiro Tohoku Pharmaceutical University Center for Labo. Anim. Sci., Lecturer, 薬学部, 講師 (30158737)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Chizuko Tohoku Pharmaceutical University Center for Labo. Anim. Sci., Assistant, 薬学部, 助手 (90296020)
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| Project Period (FY) |
2001 – 2002
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| Keywords | flexor reflex / pain / autonomic nervous system / Electrocardiogram / capsaicin / urethane anesthesia / time series analysis / PSD |
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| Research Abstract |
The flexor reflex model by noxious stimuli to a hind foot was developed using the rat under urethane anesthesia as an analysis model of a pain. Namely, the pinching stimulus to skin and the flexor reflex reaction according to capsaicin injection (CAP) into an artery generally was regarded as EMG, and it quantified by changing it into an integration value. Moreover, from the susceptibility over the local anesthetic and opioid drugs of noxious stimuli evoked reaction, it checked that it was the reaction of pain induction nature, and it was shown clearly that it is useful as an pain analysis model. When the time series analysis etc. was performed by setting the living body signal of circulatory systems, such as blood pressure, heart beats, and breathing, to parameter in order to explore change given to the autonomic nervous system of the stationary state at the time of un-stimulating in this model, and an unsteady state at the time of noxious stimuli, by the CAP injection group, the diffe
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rence was especially observed in the reaction style between these parameter(s) clearly at the time of un-stimulating and a stimulus. When power spectrum (PSD) analysis of the R-R interval of an electrocardiogram and systolic blood pressure was performed, a difference did not have private seals among both -- the peak of a low frequencies and its density are mostly in agreement with the peak which synchronized with breathing in the time of un-stimulating and a pinching stimulus. However, at the time of the CAP injection by which a strong sustained pain is evoked the fall of the power density of a peak and the rise of the power density of low frequencies, which synchronized with breathing, were observed. Blockers of sympathetic and parasympathetic nerve attenuated PSD of the low and high frequency at the time of a stimulus and un-stimulus. The CAP injected group strongly with both blockers similarly influenced PSD. This means that CAP drives the autonomic nervous system strongly. Moreover, a possibility that such a difference could be caught by the property of pain which animal receives was also suggested, and it is shown that it is effective in observation of autonomous system dynamics. Less
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Research Products
(2 results)
