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2003 Fiscal Year Final Research Report Summary

STUDIES ON KERATIN-BASED BIOMATERIALS FOR THE APPLICATION TO TISSUE ENGINEERING

Research Project

Project/Area Number 13680950
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

TANABE Toshizumi  Osaka City Univ., Graduate School of Eng., Associate Professor, 大学院・工学研究科, 助教授 (20315972)

Co-Investigator(Kenkyū-buntansha) TACHIBANA Akira  Osaka City Univ., Graduate School of Eng., Ledturer, 大学院・工学研究科, 講師 (80305614)
YAMAUCHI Kiyoshi  Osaka City Univ., Graduate School of Eng., Professor, 大学院・工学研究科, 教授 (00047325)
Project Period (FY) 2001 – 2003
KeywordsKeratin / Biocompatible Material / Tissue Engineering / Cell Scaffold / Film / Sponge / Controlled Release / Compression Molding
Research Abstract

To use wool keratins in developing tissue engineering field, we have investigated the methods to reinforce the keratin materials by mixing with other biopolymers or chemical crosslinking etc., the modification of keratin materials with bioactive molecules, and the cell adhesion and proliferation on keratin materials. The following results were obtained.
1.The composite keratin films with chitosan showed the antibacterial activity and the improved strength. In addition, chemical crosslinking of keratin with diepoxy compounds gave the highly flexible and water tolerant films.
2.Cell adhesion peptide, RODS was linked to free cysteine residues of keratin. The surface coated with RGDS-carrying keratins showed the increased cell adhesion comparing with unmodified keratins.
3.Lysozyme, as a model bioactive substance, was immobilized to free cysteine residues on freeze-dried keratin sponge. Lysozyme linked to sponge through S-C bond was stably maintained, while lysozyme linked through S-S bond was gradually released.
4.The porous keratin sponges having homogenous pore structure with a mean pore diameter of approximately 100 μm were given by freezing the keratin solution at -20℃ for 3 days followed by the lyophilization. When mouse fibroblast cells were cultivated on the sponge, the maximum cell number per area on the sponge was 37 times higher than that on commercially available culture dish.
5.To obtain a keratin film, which does not contain any additive but have high tenacity, the keratin powder prepared by spray-drying of reduced keratin solution was compression-molded. Homogenous transparent and highly strong plastic-like film was obtained by adding equal weight of 90% ethanol to keratin powder. The film had good water-tolerability and supported the cell adhesion and proliferation.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Toshizumi Tanabe: "Preparation and characterization of keratin-chitosan composite film"Biomaterials. 23. 817-825 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Tachibana: "Fabrication of wool keratin sponge scaffolds for long-term cell cultivation"J.Biotech.. 93. 165-170 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Yamauchi: "Enhanced cell adhesion on RGDS-carrying keratin film"Mater.Sci.Eng.C. 23. 467-472 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ayako Kurimoto: "Keratin Sponge : Immobilization of Lysozyme"J.Biosci.Bioeng.. 96. 307-309 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazunori Kato: "Preparation and Property of Keratin-Poly(vinyl-alcohol) Blend Fiber"J.Appl.Polym.Sci.. 91. 756-762 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazunori Kato: "Preparation and physicochemical properties of compression-molded keratin films"Biomaterials. 25. 2265-2272 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshizumi Tanabe: "Fabrication and characterization of chemically crosslinked keratin films"Mater.Sci.Eng.C. 24. 441-446 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshizumi Tanabe, Naoya Okitsu, Akira Tachibana, Kiyoshi Yamauchi: "Preparation and charecterization of keratin-chitosan composite film"Biomaterials. 23-3. 817-825 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Tachinbana, Yaunari Furuta, Hideyuki Takeshima, Toshizumi Tanabe, Kiyoshi Yamauchi: "Fabrication of wool keratin sponge scaffolds for long-term cell cultivation"J.Biotech.. 93-2. 165-170 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoshi Yamauchi, Hironobu Hojo, Yoshio Yamamoto, Toshizumi Tanabe: "Enhanced cell adhesion on RGDS-carrying keratin film"Mater.Sci.Eng.C. 23-4. 467-72 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ayako Kurimoto, Toshizumi Tanabe, Kiyoshi Yamauchi: "Keratin Sponge : Immobilization of Lysozyme"J.Biosci.Bioeng.. 96-3. 307-309 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazunori Kato, Mikio Shibayama, Toshizumi Tanabe, Kiyoshi Yamauchi: "Preparation and Property of Keratin-Poly(vinyl-alcohol) Blend Fiber"J.Appl.Polym.Sci.. 91-2. 756-762 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazunori Kato, Toshizumi Tanage, Kiyoshi Yamauchi: "Preparation and physicochemical properties of compression-moldedkeratin films"Biomaterials. 25-12. 2265-2272 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshizumi Tanabe, Naoya Okitsu, Akira Tachibana, Kiyoshi Tamauchi: "Fabrication and characterization of chemically crosslinked keratin films"Mater.Sci.Eng.C. 24-3. 41-446 (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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