2001 Fiscal Year Annual Research Report

増殖性を獲得した血管平滑筋細胞の収縮性の検討:再構成血管平滑筋標本を用いた検討

Research Project

Project/Area Number	13832006
Research Institution	Kyushu University
Principal Investigator	西村淳二九州大学, 大学院・医学研究院, 助教授 (90237727)
Co-Investigator(Kenkyū-buntansha)	平野勝也九州大学, 大学院・医学研究院, 講師 (80291516)
Keywords	血管平滑筋 / 収縮 / 細胞培養 / コラーゲンゲル / ウイルスベクター / 低分子量G蛋白
Research Abstract	本研究の目的は、増殖性を獲得した血管平滑筋細胞の収縮性変化の分子機構を明らかにすること、また、増殖性を獲得した血管平滑筋細胞の収縮性が、培養環境(温度、ホルモン、増殖因子の有無、shear stress、各種遺伝子導入)によりどの様に変化するのかを明らかにすることである。本年度の研究により以下の様なことが明らかになった。 1、50μg/mlのアスコルビン酸を培養液に加えることにより豚冠動脈平滑筋は継代数によらず収縮性を維持することができた。 2、この豚冠動脈培養平滑筋細胞とコラーゲンゲルを用いて作製した再構成血管標本は脱分極刺激、ヒスタミン、ブラジキニン、セロトニン、アンジオテンシンII、エンドセリン1により収縮を起こした。 3、また、この再構成血管標本を用いて受容体機能を保持した脱膜化標本を作製することができた。 4、バキュロウイルスにほ乳動物のプロモーターを挿入し、その下流に種々の遺伝子を組み込んだベクターを作製することに成功した。 5、このベクターを豚冠動脈培養平滑筋細胞に感染させることができた。 6、rhoAを発現させた豚冠動脈培養平滑筋細胞を用いて再構成血管標本を作製したところコントロールの2-3倍の収縮力を示した。

Research Products

(10 results)

All Other

All Publications (10 results)

[Publications] Setoguchi H, Nishimura J, Hirano K, Takahashi S, Kanaide H: "Leukoiriene C^4 enhances the contraction of porcine tracheal smooth muscle through the activation of Y-27632, a rho kinase inhibitor, sensitive pathway"Br J Pharmacol. 132. 111-118 (2001)
[Publications] Hirano K, Hirano M, Zeng Ying, Nishimura J, Kanaide H: "Cloning and functional expression of a degradation-resistant novel isoform of p27^<Kip1>"Biochem J. 353. 51-57 (2001)
[Publications] Arimura T, Suematsu N, Zhou Y, Nishimura J, Satoh S, Takeshita A, Kanaide H, Kimura A: "Identification, characterization and functional analysis of heart-specific myosin light chain phosphatase small subunit"J Biol Chem.. 276. 6073-6082 (2001)
[Publications] Ihara B, Derkach DN, Hirano K, Nishimura J, Nawata H, Kanaide H: "Ca^<2+> influx in the endothelial cells is required for the bradykinin-induced endothelium-dependent contraction in the porcine interlobar renal artery"J Physiol. 534. 701-711 (2001)
[Publications] Nakamura K, Nishimura J, Hirano K, Ibayashi S, Fujishima M, Kanaide H: "Hydroxyfasudil, an active metabolite of fasudil hydrochloride, relaxes the rabbit basilar artery by disinhibition of myosin light chain phosphatase"J Cereb Blood Flow Metab. 21. 876-885 (2001)
[Publications] Shintani Y, Hirano K, Nakayama T, Nishimura J, Nakano H, Kanaide H: "Mechanism of trypsin-induced contraction in the rat myometrium : The possible involvement of a novel member of activated receptor"Br J Pharmacol. 133. 1276-1285 (2001)
[Publications] Ieiri S, Nishimura J, Hirano K, Suita S, Kanaide H: "The mechanism for the contraction induced by Leukotrien C4 in guinea-pig taenea coli"Br J Pharmacol. 133. 529-538 (2001)
[Publications] Ohnishi Y, Hirano K, Nishuinura J, Furue M, Kanaide H: "Inhibitory effect of brefeldin A on mediated by endothelium-dependent coronary artery"Br J Pharmacol. 134. 168-178 (2001)
[Publications] Nakayama T, Hirano K, Nishimura J, Takahashi S, Kanaide H: "Mechanism of trypsin-induced endothelium-dependent vasorelaxation in the porcine coronary artery"Br J Pharmacol. 134. 815-826 (2001)
[Publications] Hirano M, Hirano K, Nishimura J, Kanaide H: "Transcriptional up-regulation of p27Kip1 during the contact-induced growth arrest in the vascular endothelial cells"Exp Cell Res.. 271. 356-367 (2001)

2001 Fiscal Year Annual Research Report

増殖性を獲得した血管平滑筋細胞の収縮性の検討:再構成血管平滑筋標本を用いた検討

Principal Investigator

西村 淳二 九州大学, 大学院・医学研究院, 助教授 (90237727)

Research Products

[Publications] Setoguchi H, Nishimura J, Hirano K, Takahashi S, Kanaide H: "Leukoiriene C^4 enhances the contraction of porcine tracheal smooth muscle through the activation of Y-27632, a rho kinase inhibitor, sensitive pathway"Br J Pharmacol. 132. 111-118 (2001)

[Publications] Hirano K, Hirano M, Zeng Ying, Nishimura J, Kanaide H: "Cloning and functional expression of a degradation-resistant novel isoform of p27^<Kip1>"Biochem J. 353. 51-57 (2001)

[Publications] Arimura T, Suematsu N, Zhou Y, Nishimura J, Satoh S, Takeshita A, Kanaide H, Kimura A: "Identification, characterization and functional analysis of heart-specific myosin light chain phosphatase small subunit"J Biol Chem.. 276. 6073-6082 (2001)

[Publications] Ihara B, Derkach DN, Hirano K, Nishimura J, Nawata H, Kanaide H: "Ca^<2+> influx in the endothelial cells is required for the bradykinin-induced endothelium-dependent contraction in the porcine interlobar renal artery"J Physiol. 534. 701-711 (2001)

[Publications] Nakamura K, Nishimura J, Hirano K, Ibayashi S, Fujishima M, Kanaide H: "Hydroxyfasudil, an active metabolite of fasudil hydrochloride, relaxes the rabbit basilar artery by disinhibition of myosin light chain phosphatase"J Cereb Blood Flow Metab. 21. 876-885 (2001)

[Publications] Shintani Y, Hirano K, Nakayama T, Nishimura J, Nakano H, Kanaide H: "Mechanism of trypsin-induced contraction in the rat myometrium : The possible involvement of a novel member of activated receptor"Br J Pharmacol. 133. 1276-1285 (2001)

[Publications] Ieiri S, Nishimura J, Hirano K, Suita S, Kanaide H: "The mechanism for the contraction induced by Leukotrien C4 in guinea-pig taenea coli"Br J Pharmacol. 133. 529-538 (2001)

[Publications] Ohnishi Y, Hirano K, Nishuinura J, Furue M, Kanaide H: "Inhibitory effect of brefeldin A on mediated by endothelium-dependent coronary artery"Br J Pharmacol. 134. 168-178 (2001)

[Publications] Nakayama T, Hirano K, Nishimura J, Takahashi S, Kanaide H: "Mechanism of trypsin-induced endothelium-dependent vasorelaxation in the porcine coronary artery"Br J Pharmacol. 134. 815-826 (2001)

[Publications] Hirano M, Hirano K, Nishimura J, Kanaide H: "Transcriptional up-regulation of p27Kip1 during the contact-induced growth arrest in the vascular endothelial cells"Exp Cell Res.. 271. 356-367 (2001)

西村淳二九州大学, 大学院・医学研究院, 助教授 (90237727)