2002 Fiscal Year Final Research Report Summary
Conversion of Agricultural Wastes to Lactic Acid by a Combination of Pentose-fermentative Lactic Acid Bacteria and Reversibly Soluble-insoluble Enzymes
| Project/Area Number |
13836003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | Niigata University |
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Principal Investigator |
TANIGUCHI Masayuki NIIGATA UNIVERSITY Faculty of Engineering, Professor, 工学部, 教授 (00163634)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Takaaki Faculty of Engineering, Associate Professor, 工学部, 助教授 (00217043)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Xylose / L-Lactic acid / Lactic acid bacteria / Agricultural waste / Biomass / Rice bran |
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| Research Abstract |
1) Glucose and xylose were mainly obtained from delignified rice straw by hydrolysis using cellulase preparation. Glucose and arabinose were mainly obtained from defatted rice bran by hydrolysis using amylase and cellulase preparations.
2) Lactobacillus vaccinostericus, Lactobacillus pentosus, Pediococcus pentosaceus, and Enterococcus casseliflavus were selected as a xylose-fermented lactic acid bacterium. More lactic acid was produced from MRS medium containing xylose by E. casseliflavus as compared with other lactic acid bacteria.
3) The amount of lactic acid produced did not increase in a fermentation using MRS medium containing xylose and glucose as a carbon source and E. casseliflavus.
4) The maintenance of pH at a low value in a simultaneous saccharification and fermentation of non-sterilized rice bran allowed the selective growth of Lactobacillus rhamnosus, a lactic acid bacterium added. About 30 g/L of L-lactic acid was produced from defatted rice bran in a simultaneous saccharification and fermentation in which the pH was kept at 4.5.
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