2003 Fiscal Year Final Research Report Summary
Isolation and application of new microorganisms from soil Samples
Project/Area Number |
13836010
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
応用微生物学
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Research Institution | Kitasato University |
Principal Investigator |
TAKAHASHI Yoko Kitasato University, Kitasato Institute for Life Sciences, Professor, 北里生命科学研究所, 教授 (80197186)
|
Co-Investigator(Kenkyū-buntansha) |
ARAI Masahoshi Kitasato University, Kitasato Institute for Life Sciences, Assistant Professor, 北里生命科学研究所, 講師 (80311231)
SHIOMI Kazuro Kitasato University, School of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (40235502)
TOMODA Hiroshi Kitasato University, Institute for Life Sciences, Professor, 北里生命科学研究所, 教授 (70164043)
|
Project Period (FY) |
2001 – 2003
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Keywords | Micobial resources / Unknown microorganisms / Superoxide / Superoxide dismutase / Catalase / New bioactive compounds / Inducers of microbial growth |
Research Abstract |
To increase the possibility of discovering useful new bioactive compounds from microorganisms, we have attempted to isolate new microbial strains by adding various substances to the isolation agar media. In the course of searching for factors necessary for bacterial colony growth, we have found that the predominant bacterial strains release proteins into the culture broths that increase the number of bacterial colonies from a soil sample. Surprisingly, the common protein isolated from the active culture troths was superoxide dismutase (SOD). Moreover, commercial SODs increased the number of colonies, a response further enhanced by the addition of catalase. Superoxide (O_2) was quantitatively detected in the isolation agar medium and the level was suppressed by adding SOD or SOD plus catalase. Our results strongly suggest that some of the bacterial cells may be recovered by suppressing oxidative stress on the agar-based medium.
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Research Products
(8 results)