2005 Fiscal Year Final Research Report Summary
Molecular anatomy of plant viral genome replication machinery for the development of cell-free viral replication system.
| Project/Area Number |
13854004
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | TAMAGAWA UNIVERSITY (2004-2005)
The University of Tokyo (2001-2003) |
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Principal Investigator |
HIBI Tadaaki Tamagawa Univ., Research Inst., Professor, 学術研究所, 教授 (50261954)
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| Co-Investigator(Kenkyū-buntansha) |
NAMBA Shigetou Univ.Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (50189221)
SHIRAKO Yukio Univ.Tokyo, Asian Natural Environmental Science Center, Professor, アジア生物資源環境研究センター, 教授 (90143023)
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| Project Period (FY) |
2001 – 2005
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| Keywords | Plant virus / RNA replication / DNA replication / Host factor / TMV / SbCMV / AGSV / SBWMV |
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| Research Abstract |
This study was intended to analyze the molecular structure of viral replication complexes (VRC) of 5 different plant viruses for developing the cell-free viral replication systems.
1.Molecular anatomy of TMV replication complex :
Tobacco mosaic virus RNA dependent RNA polymerase (RdRp) was shown to interact specifically with the host protein kinase (NtPERK1), protein phosphatase 2C (NtPP2C-2), arginine decarboxylase (ADC) and ring-finger protein (PHF15) those might regulate the viral replication. Also elongation factor eEF1A was shown to bind directly with TMV RdRp as well as 3'-UTR of TMV RNA, which indicated that eEF1A is one of the essential factors of VRC.
2.Molecular anatomy of SbCMV replication complex :
ORF II product of Soybean chlorotic mottle virus (SbCMV) showed the self-interaction that was indispensable to viral pathogenicity.
3.Molecular anatomy of ASGV replication complex :
Apple stem grooving virus (ASGV) was revealed to express its proteins through the frameshift strategy and the variable region was shown to be involved in its pathogenicity.
4.Molecular anatomy of TVX replication complex :
Potato virus X belonging to the same genus of Tulip virus X (TVX) showed that the C-terminus region of the replicase and 5'untranslated region were responsible for different types of symptoms.
5.Molecular anatomy of SBWMV replication complex and trial for developing cell-free replication system :
Replication of Soil-borne wheat mosaic virus (SBWMV) was more efficient at 17℃ than at 15℃ or 20℃. The p19 cysteine-rich protein, an RNA silencing suppressor, facilitated RNA accumulation in transfected cells late in infection as well as cell-to-cell movement of viral RNA. SBWMV RNA1 was translated in vitro in transcription-coupled wheat germ extracts using RNA1 cDNA with the upstream T7 promoter as a template. It was confirmed that translational readthrough at the UGA termination codon of the p152 gene could occur as in vivo at 24℃.
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Research Products
(41 results)
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[Journal Article] In vivo interaction between Tobacco mosaic virus RNA-dependent RNA polymerase and host translation elongation factor 1A.2006
Author(s)
Yamaji, Y., Kobayashi, T., Hamada, K., Sakurai, K., Yoshii, A., Suzuki, M., Namba, S., Hibi, T.
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Journal Title
Virology 347
Pages: 100-108
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The efficiency of interference of Potato virus X infection depends on the target gene.2006
Author(s)
Takahashi, S., Komatsu, K., Kagiwada, S., Ozeki, J., Mori, T., Hirata, H., Yamaji, Y., Ugaki, M., Namba, S.
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Journal Title
Virus Res. 116
Pages: 214-217
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Phylogenetic characteristics, genomic heterogeneity and symptomatic variation of five closely, related Japanese strains of Potato virus X.2005
Author(s)
Komatsu, K., Kagiwada, S., Takahashi, S., Mori, T., Yamaji, Y., Hirata, H., Ozeki, J., Yoshida, A., Suzuki, M., Ugaki, M., Namba, S.
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Journal Title
Virus Genes 31
Pages: 99-105
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A single amino acid residue of RNA-dependent RNA polymerase in the Potato virus X genome determines the symptoms in Nicotiana plants.2005
Author(s)
Kagiwada, S., Yamaji, Y., Komatsu, K., Takahashi, S., Mori, T., Hirata, H., Suzuki, M., Ugaki, M., Namba, S.
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Journal Title
Virus Res. 110
Pages: 177-182
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Interaction between the helicase domain of Tobacco mosaic virus replicase and a tobacco arginine decarboxylase.2004
Author(s)
Shimizu, T., Yamaji, Y., Ogasawara, Y., Hamada, K., Sakurai, K., Kobayashi, T., Watanabe, T., Hibi, T.
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Journal Title
J.Gen.Plant Pathol. 70
Pages: 353-358
Description
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[Journal Article] A single silent substitution in the Apple stem grooving virus genome causes symptom attenuation.2003
Author(s)
Hirata, H., Lu, X., Yamaji, Y., Kagiwada, S., Ugaki, M., Namba, S.
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Journal Title
J.Gen.Virol. 84
Pages: 2579-2583
Description
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[Journal Article] A single amino acid in the RNA-dependent RNA polymerase of Plantago asiatica mosaic virus contributes to systemic necrosis.
Author(s)
Ozeki, J., Takahashi, S., Komatsu, K., Kagiwada, S., Yamashita, K., Mori, T., Hirata, H., Yamaji, Y., Ugaki, M., Namba, S.
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Journal Title
Description
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