2013 Fiscal Year Annual Research Report
プルキンエ細胞の分割されたトランスラトームのデジタル表現プロファイリング
| Project/Area Number |
13F03704
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
PLESSY Charles 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー
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| Co-Investigator(Kenkyū-buntansha) |
クラッツ アントン 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員
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| Keywords | library preparation / sequencing / immunofluorescence / mass-spectrometry / sample preparation |
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| Research Abstract |
Dr. Anton Kratz has been trained to prepare and sequence nanoCAGE libraries. This will speed up the planned research considerably, as we do not need anymore to order the libraries to a third party. This also leads to a more optimal use of the research budget.
We now have mass spectrometry measurements from the first test library, and together with the first sequencing of the test library, we can start the analysis of the mRNA / protein correlations. At the same time, the experiments to prepare sample material for the main dataset, including the time course data, proceed as planned.
In addition, for a manuscript currently under review at Genome Research, we have together with our collaborators performed and analyzed immunofluorescence experiments to correlate protein and mRNA abundance in Purkinje cells. That work is essential to proceed with the current research project. This JSPS fellowship has been fully acknowledged in that manuscript.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
We conducted and analyzed an experiment about protein abundance in Purkinje cells which went into a paper describing results in previous research. This will aid in the current and future work, but slightly delayed the current research plan.
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| Strategy for Future Research Activity |
We will proceed with the research plan as outlined previously. There are no specific unforeseen difficulties ; therefore, there is no reason to change the research plan.
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Research Products
(1 results)
