2015 Fiscal Year Annual Research Report
| Project/Area Number |
13J01792
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| Research Institution | Osaka University |
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Principal Investigator |
チヤン イェ 大阪大学, 理学研究科, 特別研究員(DC1)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | Plant development / Transcription factors |
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| Outline of Annual Research Achievements |
I have been screening for the key transcription factors that determine pericycle cell Identity. My screening has revealed two clades of basic Helix-Loop-Helix (bHLH) family transcription factors, represented by No.17 (including No.17, No.17H, No.17H2, and No.17H3) and No. 51 (including No.51, No. 36, No.14, No.14L etc.), that play key roles in regulation pericycle cell identity. A Yeast-Two-Hybrid screening confirmed the interaction between the two clades of proteins.
No.51 is expressed specifically in pericycle cells. No.51 overexpression (No.51-OE) causes ectopic expression of XPP-marker GFP. Importantly, No.51-OE caused ectopic cell division in the root. Exogenous auxin increased the ectopic cell division in No.51-OE root, demonstrating that No.51 confers the competency to undergo auxin-induced cell division. No.17-OE caused similar but milder phenotype than that of No.51. No.17 is broadly expressed in several cell types including pericycle.
No.51 regulate No.17's transcription, but not vice versa. No.51 is also auto-regulated. Such feedback reinforces their own transcription. No.51’s specific expression pattern confines this network in the pericycle, which constitutes a master regulation of pericycle cell’s identity.
Transcriptional profiling of No.51-OE root shows that many pericycle-specific genes are up-regulated by No.51-OE, suggesting No.51’s role as a positive regulator of pericycle cell identity. Detailed analysis on the obtained transcriptional profile of No.51-OE/ No.51-SRDX roots will shed light on the identification of the bHLH TFs’ downstream effectors.
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| Research Progress Status |
27年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
27年度が最終年度であるため、記入しない。
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Research Products
(2 results)
