2002 Fiscal Year Annual Research Report

塩類細胞の分子解剖と分化誘導機構

Research Project

Project/Area Number	14104002
Research Institution	Tokyo Institute of Technology
Principal Investigator	広瀬茂久東京工業大学, 大学院・生命理工学研究科, 教授 (10134199)
Co-Investigator(Kenkyū-buntansha)	加藤明東京工業大学, 大学院・生命理工学研究科, 助手 (40311336) 竹井祥郎東京大学, 海洋研究所, 教授 (10129249)
Keywords	塩類細胞 / 恐山ウグイ / 酸性適応機構 / トランスポーター / RBCCタンパク質 / グルタミン代謝
Research Abstract	水生生物の存在基盤となっている塩類細胞の機能と分化誘導機構を理解するために,本年度は淡水型と海水型塩類細胞の分子解剖及び分化誘導因子の候補分子の探索を行い以下の成果を得た。1.恐山ウグイの酸性適応機構の解明:pH3.5に適応させたときにエラの塩類細胞に強<発現してくる分子群の構造・機能解析から,重炭酸イオン輸送分子で体液の酸性化を防ぐ役割を果たしているNa^+/HCO_3^-共輸送体(NBC)がこれまで知られているものに比べイオン親和性が強いなどのユニークな性質を有することを明らかにすると共に,グルタミン代謝系を活性化し,アンモニアと重炭酸イオンを生成し酸性に適応しているという驚くべき仕組みを解明した。2.淡水ウナギのエラ上皮細胞で特異的に発現するRBCCタンパク質の同定と局在部位の決定:転写調節因子様のドメイン構造を有するRING-finger B-box coiled coil (RBCC) タンパク質を同定し,エラでのみ特異的に発現し,淡水で発現量が著しく増加することを明らかにした。さらに抗体染色により塩類細胞を中心とするエラの上皮細胞の核内に多く存在することを見出した。3.淡水型塩類細胞の機能解析:過剰の塩を排出するという海水型塩類細胞の役割と分子機構の理解に比べ,淡水型塩類細胞の機能は分子レベルではほとんど分かっていない。淡水から塩を吸収するという難しい仕事を担っていると考えられているので,それらの輸送に関与する候補分子をクローニングし,機能解析の基盤を築いた。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Hirata, T. et al.: "Mechanism of acid adaptation of a fish living in a pH3.5 lake"Am. J. Physiol. Regulatory Integrative Comp. Physiol.. (印刷中). (2003)
[Publications] Hirose, S. et al.: "Natriuretic peptide receptors in the eel"Camp. Biochem. Physiol.. 134A. S45-S46 (2002)
[Publications] Miyamoto, K. et al.: "RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica"Eur. J. Biochem.. 269(24). 6152-6161 (2002)
[Publications] Nakamura, N. et al.: "Kdg1. a novel RING-finger membrane protein that localizes in the recycling endosome compartment"Mol. Biol. Cell. 13. 397a-398a (2002)
[Publications] Yada, T. et al.: "Relationships between obesity and metabolic hormones in the "cobalt" variant of rainbow trout"Gen. Comp. Endocrinol.. 128(1). 36-43 (2002)
[Publications] Miyazaki, H. et al.: "Kidney-specitic chloride channel, OmCIC-K, predominantly expressed in the diluting segment o freshwater-adapted tilapia kidney"Proc. Natl. Acad. Sci. USA. 99(24). 15782-15787 (2002)
[Publications] Anderson, W. G. et al.: "Osmotic and volaemic effects on drinking rate in elasmobranch fish"J Exp Biol.. 205(Pt8). 1115-1122 (2002)
[Publications] Tsukada, T, Takei, Y.: "Relative potency of three homologous natriuretic peptides (ANP, CNP and VNP) in eel osmoregulation"Zoolog. Sci.. 18(9). 1253-1258 (2001)
[Publications] Takei, Y., Hirose, S.: "The natriuretic peptide system in eels : a key endocrine system for euryhalinity?"Am. J. Physiol.. 282(4). R940-R951 (2002)
[Publications] Sasayama, Y. et al.: "Direct raises in blood Ca levels by intusing a high Ca solution into the blood stream accelerate the secretion of calcitonin from the ultimobranchial qland in eels"Zoolog. Sci.. 19(9). 1039-1043 (2002)
[Publications] Inoue, K., Takei, Y.: "Diverse adaptability in oryzias species to high environmental salinity"Zoolog. Sci.. 19(7). 727-734 (2002)
[Publications] Inoue, K., et al.: "Growth hormone and insulin-like growth tactor I of a euryhaline tish Cottus kazika : cDNA cloning and expression after seawater acclimation"Gen. Comp. Endocrinol.. 131(1). 77-84 (2003)
[Publications] Eckert, S.M. et al.: "Effects of anglotensin II and natriuretic peptides of the eel on prolactin and growth hormone release in the tilapia, Oreochromis mossambicus"Gen. Comp. Endocrinol.. 130(3). 333-339 (2003)
[Publications] Li, Y.Y., Takei, Y.: "Ambient salinity-dependent effects of homologous natriuretic peptides (ANP, VNP, and CNP) on plasma cortisol level in the eel"Gen. Comp. Endocrinol.. 130(3). 317-323 (2003)
[Publications] Inoue, K. et al.: "C-type natriuretic peptide of rainbow trout (Oncorhynchus mykiss) : primary structure and vasorelaxant activities"Gen. Comp. Endocrinol.. 130(3). 185-192 (2003)
[Publications] Balment, R.J. et al.: "Isolation synthesis, and biological activity of flounder [Asn(1), Ile(5), Thr(9)] angiotensin I"Gen. Comp. Endocrinol.. 130(1). 92-98 (2003)

2002 Fiscal Year Annual Research Report

塩類細胞の分子解剖と分化誘導機構

Principal Investigator

広瀬 茂久 東京工業大学, 大学院・生命理工学研究科, 教授 (10134199)

Research Products

[Publications] Hirata, T. et al.: "Mechanism of acid adaptation of a fish living in a pH3.5 lake"Am. J. Physiol. Regulatory Integrative Comp. Physiol.. (印刷中). (2003)

[Publications] Hirose, S. et al.: "Natriuretic peptide receptors in the eel"Camp. Biochem. Physiol.. 134A. S45-S46 (2002)

[Publications] Miyamoto, K. et al.: "RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica"Eur. J. Biochem.. 269(24). 6152-6161 (2002)

[Publications] Nakamura, N. et al.: "Kdg1. a novel RING-finger membrane protein that localizes in the recycling endosome compartment"Mol. Biol. Cell. 13. 397a-398a (2002)

[Publications] Yada, T. et al.: "Relationships between obesity and metabolic hormones in the "cobalt" variant of rainbow trout"Gen. Comp. Endocrinol.. 128(1). 36-43 (2002)

[Publications] Miyazaki, H. et al.: "Kidney-specitic chloride channel, OmCIC-K, predominantly expressed in the diluting segment o freshwater-adapted tilapia kidney"Proc. Natl. Acad. Sci. USA. 99(24). 15782-15787 (2002)

[Publications] Anderson, W. G. et al.: "Osmotic and volaemic effects on drinking rate in elasmobranch fish"J Exp Biol.. 205(Pt8). 1115-1122 (2002)

[Publications] Tsukada, T, Takei, Y.: "Relative potency of three homologous natriuretic peptides (ANP, CNP and VNP) in eel osmoregulation"Zoolog. Sci.. 18(9). 1253-1258 (2001)

[Publications] Takei, Y., Hirose, S.: "The natriuretic peptide system in eels : a key endocrine system for euryhalinity?"Am. J. Physiol.. 282(4). R940-R951 (2002)

[Publications] Sasayama, Y. et al.: "Direct raises in blood Ca levels by intusing a high Ca solution into the blood stream accelerate the secretion of calcitonin from the ultimobranchial qland in eels"Zoolog. Sci.. 19(9). 1039-1043 (2002)

[Publications] Inoue, K., Takei, Y.: "Diverse adaptability in oryzias species to high environmental salinity"Zoolog. Sci.. 19(7). 727-734 (2002)

[Publications] Inoue, K., et al.: "Growth hormone and insulin-like growth tactor I of a euryhaline tish Cottus kazika : cDNA cloning and expression after seawater acclimation"Gen. Comp. Endocrinol.. 131(1). 77-84 (2003)

[Publications] Eckert, S.M. et al.: "Effects of anglotensin II and natriuretic peptides of the eel on prolactin and growth hormone release in the tilapia, Oreochromis mossambicus"Gen. Comp. Endocrinol.. 130(3). 333-339 (2003)

[Publications] Li, Y.Y., Takei, Y.: "Ambient salinity-dependent effects of homologous natriuretic peptides (ANP, VNP, and CNP) on plasma cortisol level in the eel"Gen. Comp. Endocrinol.. 130(3). 317-323 (2003)

[Publications] Inoue, K. et al.: "C-type natriuretic peptide of rainbow trout (Oncorhynchus mykiss) : primary structure and vasorelaxant activities"Gen. Comp. Endocrinol.. 130(3). 185-192 (2003)

[Publications] Balment, R.J. et al.: "Isolation synthesis, and biological activity of flounder [Asn(1), Ile(5), Thr(9)] angiotensin I"Gen. Comp. Endocrinol.. 130(1). 92-98 (2003)

広瀬茂久東京工業大学, 大学院・生命理工学研究科, 教授 (10134199)