2006 Fiscal Year Final Research Report Summary
Comprehensive analysis of bone formation and its application
| Project/Area Number |
14104015
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo Medical and Dental University (2004-2006)
Nagasaki University (2002-2003) |
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Principal Investigator |
YAMAGUCHI Akira Tokyo Medical and Dental University, Graduate School, Oral Pathology, Professor, 大学院医歯学総合研究科, 教授 (00142430)
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| Co-Investigator(Kenkyū-buntansha) |
KATSUBE Ken-ichi Tokyo Medical and Dental University, Graduate School, Oral Pathology, Assistant Professor, 大学院医歯学総合研究科, 講師 (20233760)
SAKAMOTO Kei Tokyo Medical and Dental University, Graduate School, Oral Pathology, Research Associate, 大学院医歯学総合研究科, 助手 (00302886)
TAMAMURA Sadahiro Tokyo Medical and Dental University, Graduate School, Oral Pathology, Research Associate, 大学院医歯学総合研究科, 特任助手 (70431963)
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| Project Period (FY) |
2002 – 2006
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| Keywords | osteoblast / Runx2 / CCN3 / Notch / Mesenchymal stem cell / frog / evolution / bone regeneration |
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| Research Abstract |
We investigate the following issues in this research. 1)Analyses of regulatory factors of osteoblast differentiation, 2)Establishment and characterization of mesenchymal stem cells, 3)Evolutional changes of skeletal tissues. Results in this study is as follow.
1)Analyses of regulatory factors of osteoblast differentiation
a)We established runx2-deficient cell lines, and demonstrate that osteoblast differentiation is induced by BMP-2 in these cells by using other transcription factors such as Dlx5 and Msx2. b)CCN3 (NOV) inhibits osteoblast differentiation by interacting with BMP and Notch signaling pathways. c)We revealed the important role of Msx2 in osteoblast differentiation.
2)Establishment and characterization of mesenchymal stem cells
a)We established mesenchymal stem cells from human hamartoma, and characterized their differentiation potencies into various mesenchymal cells, b)We established multipotent mesenchymal cell lines form human osteosarcoma cell lines, which exhibited differ
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entiation potential into osteoblasts, chondrocytes, and adipocytes, c)We investigated the differentiation potential of multipotent KUSA cells, which were isolated from murine bone marrow, and revealed that CCN3 inhibits osteoblast differentiation in this cell line.
3)Evolutional changes of skeletal tissues
a)We demonstrated that endochondral ossification is delayed prominently in frog long bones, compared with that in mammals. This was caused by the different expression profiles of Banded Hedgehog in frog, compared with expression profiles of Indian Hedgehog in mouse long bones. b)We established cell culture methods for muscle and chondrocytes isolated frogs. c)We compared the bone regeneration process between frog and mouse. Bone regeneration process was extremely delayed, compared with that of mouse. d)We investigated whether bones in frogs play important role in calcium reservoir, and suggested that the bones in frogs will not be involved in calcium reservoir.
These results will provide important information to establish new therapies for bone regeneration and osteoporosis. Less
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[Journal Article] Regulation of osteoclast differentiation and function by the CaMK/CREB pathway.2007
Author(s)
Sato K, Suematsu A, Nakashima T, Takemoto- Kimura S, Aoki K, Morishita Y, Asahara H, Ohya K, Yamaguchi A, Takai T, Kodama T, Chatila TA, Bito H, Takayanagi H
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Journal Title
Nat Med 12
Pages: 1410-1416
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Th17 functions as an osteoclastogenic helper T cell subset that links T cell activation and bone destruction.2007
Author(s)
Sato K, Okamoto K, Suematsu A, Yamaguchi A, Morishita Y, Kadono Y, Tanaka S, Kodama T, Akira S, Iwakura Y, Cua DJ, Takayanagi H
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Journal Title
J Exp Med 203
Pages: 2673-2682
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Regulation of osteoclast differentiation and function by the CaMK/CREB pathway.2007
Author(s)
Sato K, Suematsu A, Nakashima T, Takemoto-Kimura S, Aoki K, Morishita Y, Asahara H, Ohya K, Yamaguchi A, Takai T, Kodama T, Chatila TA, Bito H, Takayanagi H
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Journal Title
Nat Med 12
Pages: 1410-1416
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] CCAAT/enhancer binding protein β isoform, LIP, regulates commitment of osteoblasts and adipocytes.2005
Author(s)
Hata K, Nishimura R, Ueda M, Ikeda F, Matsubara T, Ichida F, Hisada K, Nokubi T, Yamaguchi A, Yoneda T
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Journal Title
Mol Cell Biol. 25
Pages: 1971-1979
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Reciprocal roles of Msx2 in regulation of osteoblast and adipocyte differentiation.2004
Author(s)
Ichida F, Nishimura R, Hata K, Matsubara T, Ikeda F, Hisada K, Yatani H, Cao X, Komori T, Yamaguchi A, Yoneda T
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Journal Title
J Biol Chem 279
Pages: 34015-34022
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Transplantation of skin fibroblasts expressing BMP-2 promotes bone repair more effectively than those expressing Runx2.2003
Author(s)
Hirata K, Tsukazaki T, Kadowaki A, Furukawa K, Shibata Y, Moriishi T, Okubo Y, Bessho K, Komori T, Mizuno A, Yamaguchi A
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Journal Title
Description
「研究成果報告書概要(和文)」より
