2004 Fiscal Year Final Research Report Summary
Diagnosis, epidemiology and prevention of flavivirus infection including West Nile fever.
| Project/Area Number |
14206036
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKASHIMA Ikuo Hokkaido University, Graduate School of Veterinary Medicine, Professor, 大学院・獣医学研究科, 教授 (30002083)
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| Co-Investigator(Kenkyū-buntansha) |
KARIWA Hiroaki Hokkaido University, Graduate School of Veterinary Medicine, Associate Professor, 大学院・獣医学研究科, 助教授 (70224714)
MORITA Koichi Institute for Tropical Medicine, Nagasaki University, Professor, 熱帯医学研究所, 教授 (40182240)
TADANO Masayuki Medical School, Ryuku University, Associate Professor, 医学部, 助教授 (80179712)
TAKEGAMI Tsutomu Kanazawa Medical Schools, Institute for Medical Sciences, Professor, 総合医学研究所, 教授 (10113490)
ESHITA Yuki Ohita Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10082223)
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| Project Period (FY) |
2002 – 2004
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| Keywords | West Nile fever / Flavivirus infection / diagnosis / epidemiology |
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| Research Abstract |
In order to prevent the outbreaks of flavivirus infections, we developed the diagnostic tests, examined pathogenecity of the viruses and examined vector susceptibility of the Japanese mosquitoes. The results are summarized as follows.
1.Development of diagnostic tests.
1)As the genetic diagnostic tests of West Nile virus, RT-PCR RFLP method, a real time PCR method and RT-LAMP method were developed.
2)For tick-borne encephalitis, IgG-and IgM-ELISA using subviral particles were developed as serological diagnostic tests for human.
2.Studies on pathogenecity of flavivirus
1)Neuroinvasive virulence of West Nile virus New York strain was found to be associated with glysosylation of envelope protein.
2)Neuroinvasive virulence of tick-borne encephalitis virus was reduced due to one amino acid change of envelope protein which led to the reduced vircemia.
3.Japanese encephalitis
1)Japanese encephalitis virus from South-East Asian countries often invaded into Japan.
2)RNAi was found to be effective as antiviral agent to Japanese encephalitis viris.
4.Mosquito studies
1)Japanese indigenous mosquitoes of 4 species were found to be susceptible to West Nile virus.
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[Journal Article] Amino acid chages responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of Tick-borne encephalitis virus.2004
Author(s)
Hayasaka, D., Gritsun, T.S, Yoshii, K., Ueki, T., Goto, A., Mizutani, T., Kariwa, H., Iwasaki, T., Gould, E.A., Takashima, I.
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Journal Title
J Gen Virol. 85
Pages: 1007-1018
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion.2004
Author(s)
Yoshii, K., Konno, A., Goto, A., Nio, J., Obara, M., Ueki, T., Hayasaka, D., Mizutani, T., Kariwa, H., Takashima I
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Journal Title
J Gen Virol. 85
Pages: 3049-3058
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Shift in Japanese encephalitis virus (JEV) genotype circulating in northrn Vietnam : implications for frequent introductions of JEV from Southeast Asia to East Asia.2004
Author(s)
Nga, P.T., Parquet, M.C., Cuong, V.D., Ma, S-P., Hasebe, F., Inoue, S., Makino, Y., Takagi, M., Nam, V.S., Morita, K.
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Journal Title
J Gen Virol. 85
Pages: 1625-1631
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fusion PCR generated Japanese encephalitis virus/dengue 4 virus chimera exhibits lack of neuroinvasiveness, attenuated neurovirulence, a dual-flavi immune response in mice.2004
Author(s)
Mathenge, E.G.M., Parquet, M.C., Funakoshi, Y., Houhara, S., Wong P.F., Ichinose, A., Hasebe, F., Inoue, S., Morita, K.
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Journal Title
J Gen Virol. 85
Pages: 2503-2513
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Seroepidemiology of dengue and assessment of public awareness in the Dcominican Republic.2004
Author(s)
Makino, Y., Shichijo, A., Bello, C., Eshita, Y., Disla, M., Cesin, A.J., Galcla, B., Lora, M., Valdez, S., Aquino, J.D., Aono, H., Ma, S-P., Takeshita, M.
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Journal Title
Tropical Med and Health. 32
Pages: 305-309
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus.2004
Author(s)
Shirato, K., Miyoshi, H, Goto, A., Ako, Y., Ueki, T., Kariwa,.H, Takashima, I.
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Journal Title
J Gen Virol 85
Pages: 3637-3645
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Enzyme-linked immunosorbent assay using recombinant antigens expressed in mammalian cells for serodiagnosis of tick-borne encephalitis.2003
Author(s)
Yoshii, K., Hayasaka, D., Goto, A., Obara, M., Araki, K., Yoshimatsu, K., Arikawa, J., Ivanov, L.I., Mizutani, T., Kariwa, H., Takashima, I.
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Journal Title
J.virol.Methods 108(2)
Pages: 171-179
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of JNK-like protein derived from a mosquito cell line, C6/36.2003
Author(s)
Mizutani, T., Kobayashi, M., Eshita, Y., Inanami, O., Yamamori, T., Goto, A., Miyoshi, H., Miyamoto, H., Kariwa, H., Kuwabara, M, Takashima, I.
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Journal Title
Ins Mol Biol. 12
Pages: 61-66
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Distribution of three arbovirus antibodies among monkeys (Macaca fascicularis)in the Philippines.2003
Author(s)
Inoue, S., Morita, K., Matias, R.R., Tuplano, J.V., Resuello, R.R.G., Gandelario, J.R., Cruz, D.J.M., Mapua, C.A., Hasebe, F., Igarashi, A., Natividad F.F.
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Journal Title
J.Med.Primatology. 32
Pages: 89-94
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Serodiagnosis of Japanese encephalitis among Nepalese patients by the particle agglutination assay.2003
Author(s)
Pandey, B., Yamamoto, A., Marita, K., Kurosawa, Y., Rat, S., Adhikari, S., Kandel, P., Kurane, I.
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Journal Title
Epidemiol.Infect. 131
Pages: 881-885
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia. I.2002
Author(s)
Goto, A., Hayasaka, D., Yoshii, K., Mizutani, T., Kariwa, H, Takashima, I.
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Journal Title
Jpn.J.Vet.Res. 49
Pages: 297-307
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Infection and dissemination of two dengue type-2 viruses isolated from patients exhibiting different disease severity in orally infected Aedes aegypti from different geographic origin.2002
Author(s)
R.E.Morales, Marita, K., Eshita, Y., Tsuda, Y., Fukuma, T., M, Takagi.
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Journal Title
Med.Entomol.Zool. 53
Pages: 21-27
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Involvement of the JNK-like protein of the Aedes albopictus mosquito cell line, C6/36, in phagocytosis, endocytosis and infection of West Nile virus.2000
Author(s)
Mizutani, T., Kobayashi, M., Eshita, Y., Shirato, K., Kimura, T., Ako, Y., Miyoshi, H., Takasaki, T., Kurane, I., Kariwa, H, Umemura, T., Takashima, I.
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Journal Title
Ins Mol Biol. 12
Pages: 491-499
Description
「研究成果報告書概要(欧文)」より
