2005 Fiscal Year Final Research Report Summary
Analysis on cell-to-cell interaction in gastroenteral cancer-Impact of epigenetic change of cancer cells as one of the directions of researches in post-genome era
| Project/Area Number |
14207050
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Gunma University |
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Principal Investigator |
KUWANO Hiroyuki Gunma University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (90186560)
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| Co-Investigator(Kenkyū-buntansha) |
ASAO Takayuki Gunma University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (40212469)
KATO Hiroyuki , 医学部, 講師 (70224532)
SHIMURA Tatsuo , 医学部, 助手 (00282393)
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| Project Period (FY) |
2002 – 2005
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| Keywords | gastroenteral cancer / epigenetic change / cell-to-cell interaction / metastatic potential / invasive potential / post-genome |
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| Research Abstract |
1.Four kinds of human cancer cell lines and one mouse cancer cell line were inoculated into the subepithelial area of the anorectum of female nude mice. Among the cell lines, two cell lines (KATO III and Lu 135) showed the potential enforcement of atypical changes in the adjacent mouse anorectal epithelium. Moreover, the submucosal invasion of the malignant transformed cells of the mouse epithelium was demonstrated in specimens obtained from three KATO III-inoculated mice. This exciting and novel phenomenon clearly demonstrates the need to change the present general concept of a single-cell origin of cancer tissue. This valuable and novel discovery may change the basis of oncology research while also providing new ideas for projects to investigate the mechanisms of carcinogenesis from several aspects such as molecular biology, cell biology, and pathology. Moreover, the novel experimental design itself is also extremely useful as a simple model for investigating the mechanisms of oncoge
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nesis.
2.Although cell density in cultured cells has demonstrated several alterations in the nature of cell kinetics, the changes in the metastatic aggressiveness of cancer lines under different cell densities have not yet been studied. METHODS : In the current study, we investigated the influence of changing the cell density of cultured cancer cells (colon 26 and B16-F10) injected into the tail vein in BALB/c mice on the metastatic activity by evaluating the number of lung metastases, and the possible mechanisms of this phenomenon were discussed based on the basis of the results of an invasion assay and a cell adhesion assay. RESULTS : The number of metastatic nodules was significantly higher in the high-density group than in the low one in colon 26 (p<0.005), however, this phenomenon was not seen in B16-F10. Next, we performed the same experiment by changing the environment to the opposite conditions for the cells in the low- and high-density groups, and the results showed the metastatic activities to be always higher in the high-density group. Moreover, although no difference was seen regarding the invasive activity between the high- and low-density groups, an adhesion assay showed the difference in the adhesion cell rate to be significantly higher in the high-density group especially in early period after coculture with human umbilical vein endothelial cells (HUVEC) (p<0.05). CONCLUSION : In some cell types, the metastatic activity could be altered and reversed by changing the environment, such as the cell density, during a relatively short period. As a result, the epigenetic changes of cancer cells are thus suggested to play a certain role in the malignant potentiality. Less
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Research Products
(78 results)
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[Journal Article] A novel technique to facilitate laparoscopic repair of large paraesophageal hernias.2006
Author(s)
Kato H, Miyazaki T, Kimura H, Faried A, Sohda M, Nakajima M, Fukai Y, Masuda N, Fukuchi M, Manda R, Ojima H, Tsukada K, Kuwano H.
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Journal Title
Am J Surg. 191(4)
Pages: 545-548
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Comparison between whole-body positron emission tomography and bone scintigraphy in evaluating bony metastases of esophageal carcinomas.2005
Author(s)
Kato H, Miyazaki T, Nakajima M, Takita J, Kimura H, Faried A, Sohda M, Fukai Y, Masuda N, Fukuchi M, Manda R, Ojima H, Tsukada K, Kuwano H, Oriuchi N, Endo K.
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Journal Title
Anticancer Res. 25(6C)
Pages: 4439-4444
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Classification of recurrent esophageal cancer after radical esophagectomy with two- or three-field lymphadenectomy.2005
Author(s)
Kato H, Fukuchi M, Miyazaki T, Nakajima M, Kimura H, Faried A, Sohda M, Fukai Y, Masuda N, Manda R, Ojima H, Tsukada K, Kuwano H.
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Journal Title
Anticancer Res. 25(5)
Pages: 3461-3467
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Serum p53 antibodies in drainage blood from the azygos vein in patients with esophageal carcinoma.2005
Author(s)
Kato H, Nakajima M, Fukuchi M, Miyazaki T, Manda R, Kimura H, Faried A, Sohda M, Fukai Y, Masuda N, Tsukada K, Kuwano H.
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Journal Title
Anticancer Res. 25(5)
Pages: 3231-3235
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The incremental effect of positron emission tomography on diagnostic accuracy in the initial staging of esophageal carcinoma.2005
Author(s)
Kato H, Miyazaki T, Nakajima M, Takita J, Kimura H, Faried A, Sohda M, Fukai Y, Masuda N, Fukuchi M, Manda R, Ojima H, Tsukada K, Kuwano H, Oriuchi N, Endo K.
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Journal Title
Cancer.1 103(1)
Pages: 148-156
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The effectiveness of planned esophagectomy neoadjuvant chemoradiotherapy for advanced carcinomas.2004
Author(s)
Kato H, Fukuchi M, Manda R, Faried A, Takita J, Nakajima M, Miyazaki T, Sohda M, Fukai Y, Masuda N, Tsukada K, Kuwano H.
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Journal Title
Anticancer Res. 24(6)
Pages: 4091-4096
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cell density modulates the metastatic aggressiveness of a mouse colon cancer cell line, colon 26.2004
Author(s)
Kuwano H, Miyazaki T, Tsutsumi S, Hirayama I, Shimura T, Mochiki E, Nomoto K, Fukuchi M, Kato H, Asao T.
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Journal Title
Oncology 67(5-6)
Pages: 441-449
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A new proliferation marker, minichromosome maintenance protein 2, is associated with tumor aggressiveness in esophageal squamous cell carcinoma.2003
Author(s)
Kato H, Miyazaki T, Fukai Y, Nakajima M, Sohda M, Takita J, Masuda N, Fukuchi M, Manda R, Ojima H, Tsukada K, Asao T, Kuwano H.
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Journal Title
J Surg Oncol 84(1)
Pages: 24-30
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sentinel lymph nodes with technetium-99m colloidal rhenium sulfide in patients with esophageal carcinoma.2003
Author(s)
Kato H, Miyazaki T, Nakajima M, Takita J, Sohda M, Fukai Y, Masuda N, Fukuchi M, Manda R, Ojima H, Tsukada K, Asao T, Kuwano H, Oriuchi N, Endo K.
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Journal Title
Cancer.1 98(5)
Pages: 932-939
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Correlation of 18-F-fluorodeoxyglucose (FDG) accumulation with glucose transporter (Glut-1) expression in esophageal squamous cell carcinoma.2003
Author(s)
Kato H, Takita J, Miyazaki T, Nakajima M, Fukai Y, Masuda N, Fukuchi M, Manda R, Ojima H, Tsukada K, Kuwano H, Oriuchi N, Endo. K.
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Journal Title
Anticancer Res. 23(4)
Pages: 3263-3272
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Glut-1 glucose transporter expression in esophageal squamous cell carcinoma is associated with tumor aggressiveness.2002
Author(s)
Kato H, Takita J, Miyazaki T, Nakajima M, Fukai Y, Masuda N, Fukuchi M, Manda R, Ojima H, Tsukada K, Kuwano H.
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Journal Title
Anticancer Res. 22(5)
Pages: 2635-2639
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Usefulness of positron emission tomography for assessing the response of neoadjuvant chemoradiotherapy in patients with esophageal cancer.2002
Author(s)
Kato H, Kuwano H, Nakajima M, Miyazaki T, Yoshikawa M, Masuda N, Fukuchi M, Manda R, Tsukada K, Oriuchi N, Endo K.
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Journal Title
Am J Surg. 184(3)
Pages: 279-283
Description
「研究成果報告書概要(欧文)」より
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