2004 Fiscal Year Final Research Report Summary
Compulsory ordination of biological cells in wave-guide long-path optical cell for detection of trace components in biological cells.
| Project/Area Number |
14340235
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
分離・精製・検出法
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
FUJIWARA Kitao Tokyo University of Pharmacy and Life Science, School of Life Science, Professor, 生命科学部, 教授 (90090521)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Tatsuya Tokyo University of Pharmacy and Life Science, School of Life Science, Associate Professor, 生命科学部, 助教授 (30261548)
SAKURAI Teruaki Tokyo University of Pharmacy and Life Science, School of Life Science, Lecturer, 生命科学部, 講師 (30266902)
KUMATA Hidetoshi Tokyo University of Pharmacy and Life Science, School of Life Science, Assistant Professor, 生命科学部, 助手 (60318194)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Chlorophyl / Fluorometry / Wave-guide phenomena / Superoxide anion / Autocorrelation function / Micro-algae / Metal poison / Solar-induced chemical reaction |
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| Research Abstract |
1.Teflon AF 2400 was recently commercialized, that has a refractive index(1.29) lower than water (1.33), and which means that the wave-guiding of light is possible in water. We used Teflon AF 2400 as a wave-guide capillary longpath cell for fluorometry. He-Cd and Ar lasers were used as the excitation source, at 325 nm and 514.5 nm, respectively. The excitation was executed through the wave-guide cell and the fluorescence from the wound capillary cell was collected in a perpendicular direction to the loop. With excitation at 325 nm, the fluorescence intensity at 450 nm emitted from the cell wall decreased along with the increase in the refractive index of the solvent. This can be caused by attenuation of the source light due to absorption by the solvent. In our experiment, the solvent of the higher refractive index has the higher absorption at 325 nm. On the other hand, the fluorescence intensity at 590 nm, with excitation at 514.5 nm, increases with refractive index of the solvent.
2.Th
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e wave-guide cell was used for irradiation of solar light in the solution. In connection with the photo-chemical processes in the aquatic environment, the evaluation system for the production ability of superoxide anion was constructed. The technique was based on the flow injection method with luminol chemiluminescence detection. After irradiation was finished, the carrier (with sample) flow was merged with the luminol solution and was introduced into a chemiluminescence detector. The results of the laboratory experiment show that the production of superoxide is linearly related to the concentration of humic acid up to 50 ppm. By means of changing the flow rate of the carrier, the half-life time of superoxide at pH 11 aqueous solution was estimated as 15 s. ESR spectra were obtained after 5 min. irradiation of the solar simulator. In addition to the four sharp peaks due to OH radicals, a broad peak appeared at the middle of the OH signal. The river water was collected at 18 points of the Tamagawa River located in Tokyo. Upstream, the production ability of superoxide was observed, but not downstream or in the estuarine district.
3.When heavy metal ions such as Zn(II), Ni(II) and PT(IV), Chlorella kessleri (minute unicellular algae) transformed to a large connected cell (not-devided). The regular type and the large connected type cell was introduced into the wave-guide cell mentioned in 1. The auto-correlation function was taken from the fluorescence of the wave-guide capillary, and some difference based on the cell shape was obtained. Less
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Research Products
(21 results)
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[Journal Article] Evaluation of in vitro acute immunotoxicity of arsenocholine, a trimethyl arsenic compound in seafood.2005
Author(s)
Sakurai, T., Ochiai, M., Kojima, C., Ohta, T., Fujiwara, K.
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Journal Title
Appl.Organomet.Chem. 19
Pages: 226-230
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Flow injection analysis to measure the production ability of superoxide with chemiluminescence detection in natural water.2005
Author(s)
Fujiwara, K., Kumata, H., Kando, N., Sakuma, E., Aihara, M., Morita, Y., Miyakawa, T.
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Journal Title
Int.J.Environ.Anal.Chem. 85(in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cellular glutathione prevents cytolethality of monomethylarsonic acid.2004
Author(s)
Sakurai, T., Kojima, C., Ochiai, M., Ohta, T., Sakurai, M.H., Waalksa, M.P., Fujiwara, K.
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Journal Title
Toxycology and Appl.Pharmacol. 195
Pages: 129-141
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Role of glutathione in dimethylarsinic acid-induced apoptosis.2004
Author(s)
Sakurai, T., Ochiai, M., Kojima, C., Ohta, T., Sakurai, M.H., Takada, N.O., Qu, W., Waalkes, M.P., Fujiwara K.
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Journal Title
Toxycology and Appl.Pharmacol. 198
Pages: 354-365
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Evaluation of immunotoxic and immunodisruptive effects of inorganic arsenite on human/macrophages.2004
Author(s)
Sakurai, T., Ohta, T., Tomita, N., Kojima, C., Hariya, Y., Mizukami, A., Fujiwara, K.
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Journal Title
Internat.Immunopharmacol. 4/3
Pages: 1661-1673
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Evaluation of immunotoxic effects of arsenic and other trace elements on human peripheral blood monocytes.2004
Author(s)
Sakurai T., Tomita, N., Ohta, T., Kojima, C., Hariya, Y., Mizukami, A., Fujiwara, K.
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Journal Title
Biomed.Res.Trace Elements 15
Pages: 259-261
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Study of in vitro cytotoxicity of arsenocholine, a trimethyl arsenic compound in seafood.2002
Author(s)
Sakurai, T., Ochiai, M., Kojima, C., Kumata, H., Fujiwara, K.
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Journal Title
Appl.Organomet.Chem. 16(8)
Pages: 415-420
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cytotoxicological aspects of the organic arsenic compound arsenobetaine in marine animals.2002
Author(s)
Kojima, C., Sakurai, T., Ochiai, M., Kumata, H., Qu, W., Waalkes, M.P., Fujiwara, K.
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Journal Title
Appl.Organomet.Chem. 16(8)
Pages: 421-426
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Benzothiazolamines (BTs) as tire derived molecular markers : Sorptive behavior in street runoff and application to source apportioning.2002
Author(s)
Kumata, H., Yamada, J., Masuda, K., Takada, H., Sato, Y., Fujiwara, K.
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Journal Title
Environ.Sci.Technol. 36
Pages: 702-708
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Study of in vitro cytotoxicity of arsenocholine, a trimethyl arsenic compound in seafood.2002
Author(s)
Sakurai, T., Ochiai, M., Kojima, C., Kumata, H., Fujiwara, K.
-
Journal Title
Appl.Organomet.Chem. 16
Pages: 415-420
Description
「研究成果報告書概要(欧文)」より
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