2003 Fiscal Year Final Research Report Summary
Research for the genetic mechanism of reproductive isolation between two mouse subspecies
| Project/Area Number |
14340239
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
遺伝
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
SHIROISHI Toshihiko National Institute of Genetics, Mammalian Genet.Lab., Prof, 系統生物研究センター, 教授 (90171058)
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| Project Period (FY) |
2002 – 2003
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| Keywords | hybrid breakdown / X chromosome / consomic strain / male sterility / spermatogenesis |
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| Research Abstract |
Hybrid breakdown is a type of reproductive failure that appears after the F2 generation of crosses between different species or subspecies. It is caused by incompatibility between interacting genes. Genetic analysis of hybrid breakdown, particularly in higher animals : has been hampered by its complex nature (i.e., it involves more than two genes, and the phenotype is recessive). We studied hybrid breakdown using a new consomic strain, C57BU6J-X^<MSM> in which the X chromosome of C57BL/6J (derived mostly from Mus musculus domesticus) is substituted by the X chromosome of the MSM/Ms strain (M.m.molossinus). Males of this consomic strain are sterile, whereas F_1 hybrids between C57BU6J and MSM/Ms are completely fertile. The C57BU6J-X^<MSM> males showed reduced testis weight with variable defects in spermatogenesis and abnormal sperm head morphology. We conducted quantitative trait locus (QTL) analysis for these traits to map the X-linked genetic factors responsible for the sterility. This analysis successfully detected at least three distinct loci for the sperm head morphology and one for the testis weight. This study revealed that incompatibility of interactions of X-linked gene(s) with autosomal and/or Y-linked gene(s) causes the hybrid breakdown between the genetically distant C57BL/6J and MSM/Ms strains.
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Research Products
(2 results)
