2005 Fiscal Year Final Research Report Summary
Involvement of annexin 5 in G protein mediated signal transduction
| Project/Area Number |
14360181
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Kitasato University |
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Principal Investigator |
KAWAMINAMI Mitsumori Kitasato University, Professor, 獣医畜産学部, 教授 (00153007)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Junichiro National Institute of Biomedical Innovation, Head of laboratory, 生物資源研究部・実験動物開発研究室, 研究リーダー (60181731)
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| Project Period (FY) |
2002 – 2005
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| Keywords | G protein / annexin 5 / GnRH / LH βsubunit / pituitary / gonadotrope / corpus luteum / testis |
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| Research Abstract |
A new G protein mediated signal transduction mechanism was studied. It was clarified that annexin 5 took part in the G protein coupled signal transduction process. Annexin is a member of newly identified calcium-phospholipid binding proteins. The outline of the results is brought together as follows.
1.From GnRH receptor to annexin 5 synthesis
It was clarified that the MAPK system lay between the GnRH receptor and annexin 5 mRNA synthesis in the pituitary gonadotropes. In addition, it was observed that annexin 5 was indispensable to the process that GnRH promotes the gonadotrophin secretion, and clarified that annexin 5 was a new signal factor of the GnRH receptor signal transduction.
2.Annexin 5 in the ovary
Changes in ovarian annexin 5 were analyzed. The synthesis of annexin 5 was demonstrated to start after luteinization and the expression of annexin 5 was inhibited by prolactin. It was clarified that annexin 5 appeared in corpus luteum by the inhibition of prolactin secretion and those cells were in apoptosis. In addition, it was clarified that local GnRH enhanced annexin 5 expression and induced apoptosis.
3.Annexin 5 in the testis
Annexin 5 was demonstrated to distribute to various testicular cells. Among these, endotherial cells and Leydig dells were intensely stained with anti-annexin 5. It was clarified that annexin 5 of Leydig cells was increased by the administration of human chorinic gonadotropin. This effect was shown to be mediated by the local GnRH action.
Getting together, annexin 5 was shown to be under the control of GnRH receptor in the anterior pituitary gonadotropes, luteal cells and Leydig cells. Annexin 5 is a biomarker of GnRH receptor. GnRH receptor-annexin 5 system seems to be utilized in various cell species.
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Research Products
(32 results)
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[Journal Article] Skeletal muscle FOXO1 (FKHR)-transgenic mice have less skeletal muscle mass, down-regulated type I (slow twitch/red muscle) fiber genes, and impaired glycemic control2004
Author(s)
Kamei Y, Miura S, Suzuki M, Kai Y, Mizukami J, Taniguchi T, Mochida K, Hata T, Matsuda J, Aburatani H, Nishino I, Ezaki O
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Journal Title
J Biol Chem 279
Pages: 41114-41123
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Chemical chaperone therapy for brain pathology in GM1-gangliosidosis2003
Author(s)
Matsuda J, Suzuki O, Oshima A, Yamamoto Y, Noguchi A, Takimoto K, Itoh M, Matsuzaki Y, Yasuda Y, Ogawa S, Sakata Y, Nanba E, Higaki K, Ogawa Y, Tominaga L, Ohno K, Iwasaki H, Watanabe
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Journal Title
Proc Natl Acad Sci USA 100
Pages: 15912-15917
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Regulatory sequence elements of mouse GLUT4 gene expression in adipose tissues2003
Author(s)
Miura S, Tsunoda N, Ikeda S, Kai Y, Ono M, Maruyama K, Takahashi M, Mochida K, Matsuda J, Lane MD, Ezaki O
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Journal Title
Biochem Biophys Res Commun 312
Pages: 277-284
Description
「研究成果報告書概要(欧文)」より
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