2004 Fiscal Year Final Research Report Summary
Studies on epidemiology and pathogenesis of emerging tick-borne rickettsial pathogens in small animals.
| Project/Area Number |
14360190
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Yamaguchi University |
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Principal Investigator |
INOKUMA Hisashi Yamaguchi University, Faculty of Agriculture, Professor, 農学部, 教授 (70263803)
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| Co-Investigator(Kenkyū-buntansha) |
OKUDA Masaru Yamaguchi University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (10325243)
HOSOI Eiji Yamaguchi University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60263802)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Ticks / Rickettsia / Ehrlichia / Anaplasma / Dogs / Zoonosis |
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| Research Abstract |
The research was focused on the epidemiology and pathogenesis of emerging tick-borne rickettsial pathogens related to small animals. Two results were obtained as follows ;
1.Epidemiology of emerging tick-borne rickettsial : Ticks recovered from more than 1400 dogs and cats from all over Japan were examined for several tick-borne rickettsial by using molecular methods. (1) A novel Ehrlichia sp. was detected from ticks in mainland in Japan. (2) Four tick samples from Fukushima, Miyazaki and Kagoshima Prefectures recovered from 4 different dogs showed a positive reaction for Anaplasma platys. It was the first detection of the pathogen in mainland. (3) Several new species of spotted fever group rickettsia were detected from ticks in all over Japan. (4) Hemoplasma was detected from ticks recovered from dogs and cats.
2.Establishment of diagnostic method for a novel Ehrlichia sp. detected from Ixodes ovatus : (1) Immunofluoressence assay to detect antibodies against the pathogens was established. By using this method, several dogs showed positive against the pathogens, although relationship between antibody titers and clinical symptoms were not clear. (2) Experimental inoculation of the pathogens into dogs revealed that the pathogen cause thrombocytopenia and splenomegary to the canine host. However, severe clinical symptoms were not recorded. Antibodies against the pathogen were also detected after inoculation. And the antibodies also reacted with Ehrlichia canis antigens, which is a more pathogenic agent to canine hosts.
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Research Products
(34 results)
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[Journal Article] Detection of ehrlichial DNA in Haemaphysalis ticks recovered from dogs in Japan that is closely related to a novel Ehrlichia sp. found in cattle ticks from Tibet, Thailand and Africa.2004
Author(s)
Inokuma, H., Beppu, T., Okuda, M., Shimada, Y, Sakata, Y.
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Journal Title
J.Clin.Microbiol. 42
Pages: 1353-1355
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular survey of Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemofelis' in cats in Yamaguchi and surrounding area.2004
Author(s)
Inokuma, H., Taroura, S., Okuda, M., Hisasue, M., Itamoto, K., Une, S., Nakaichi, M., Taura, Y.
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Journal Title
J.Vet.Med.Sci. 66
Pages: 1017-1020
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A case of Haemobartonellosis following splenectomy in a dog.2004
Author(s)
Hiraoka, H., Miyama, T., Shiranaga, N., Nakaichi, M., Watanabe, M., Itamoto, K., Okuda, M., Inokuma, H.
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Journal Title
J.Jpn.Vet.Med.Assoc. 57
Pages: 587-590
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Tick paralysis by Ixodes holocyclus in a Japanese traveler returning from Australia associated with Rickettsia helvetica infection.2003
Author(s)
Inokuma, H., Takahata, H., Fournier, P-E., Brouqui, P., Raoult, D., Okuda, M., Onishi, T., Nishioka, K., Tsukahara, M.
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Journal Title
J.Travel Med. 10
Pages: 61-63
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ixodid tick species recovered from domestic dogs in Japan.2003
Author(s)
Shimada, Y, Beppu, T., Inokuma, H., T., Okuda, M., Onishi, T.
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Journal Title
Med.Vet.Entomol. 17
Pages: 38-45
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Tick paralysis by Ixodes holocyclus in a Japanese traveler returning from Australia.2003
Author(s)
Inokuma, H., Takahata, H., Fournier, P-E., Brouqui, P., Raoult, D., Okuda, M., Onishi, T., Nishioka, K., Tsukahara, M.
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Journal Title
Ann.N.Y.Acad.Sci. 990
Pages: 357-358
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Epidemiological survey of Babesia species in Japan performed with specimens from ticks collected from dogs and detection of new Babesia DNA closely related to Babesia odocoilei and Babesia divergens.2003
Author(s)
Inokuma, H., Yoshizaki, Y., Shimada, Y., Sakata, Y, Okuda, M., Onishi, T.
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Journal Title
J.Clin.Microbiol. 41
Pages: 3494-3498
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] First Evidence of Anaplasma platys in Rhipicephalus sanguineus (Acari, Ixodida) collected in Africa.2003
Author(s)
Sanogo, Y.O., Davoust, B., Inokuma, H., Camicas, J-L., Parola, P., Brouqui, P.
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Journal Title
Ondersterpoort J.Vet.Res. 70
Pages: 205-212
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Culture and phenotypic characterization of a Wolbachia pipientis isolate.2003
Author(s)
Fenollar, F., La Scola, B., Inokuma, H., Dumler, J.S., Taylor, M.J., Raoult, D.
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Journal Title
J.Clin.Microbiol. 41
Pages: 5434-5441
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Determination of nucleotide sequences of heat shock operon groESL and the citrate synthase gene (gltA) of Anaplasma (Ehrlichia) platys for phylogenetic and diagnostic studies.2002
Author(s)
Inokuma, H., Fujii, K., Okuda, M., Onishi, T., Beaufils, J-P., Raoult, D., Brouqui, P.
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Journal Title
Clin.Diag.Labo.Immunol. 9
Pages: 1132-1136
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Anaplasma platys (Ehrlichia platys) infection in a dog in France : Description of the case, and characterization of the agent.2002
Author(s)
Beaufils, J-P., Inokuma, H., Brouqui, P., Martin-Garnel, J., Jumelle, Ph., Barbault- Jumelle, M.
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Journal Title
Revue Med.Vet. 153
Pages: 85-90
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Demonstration of Anaplasma (Ehrlichia) platys inclusions in peripheral blood platelets of a dog in Japan.2002
Author(s)
Inokuma, H., Fujii, K., Matsumoto, K., Okuda, M., Nakagome, K., Kosugi, R., Hirakawa, M., Onishi, T.
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Journal Title
Vet.Parasitol. 110
Pages: 145-152
Description
「研究成果報告書概要(欧文)」より
