Generation mechanisms of nitrogen dioxide-like species from cardiovascular system.

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 14370120
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Kanazawa University
• Principal Investigator: OGINO Keiki Kanazawa University, Graduate School of Medical Science, Professor, 医学系研究科, 教授 (70204104)
• Co-Investigator(Kenkyū-buntansha): KAMBAYASHI Yasuhiro Kanazawa University, Graduate School of Medical Science, Lecturer, 医学系研究科, 講師 (20345630) ; KATO Masashi Chubu University, Professor, 生命健康科学研究所, 教授 (10281073) ; NAKAMURA Hiroyuki Kochi Medical School, Professor, 医学部, 教授 (30231476) ; NOBUKUNI Yoshitaka Kanazawa University, Graduate School of Medical Science, Lecturer, 医学系研究科, 講師 (80295641)
• Project Period (FY): 2002 – 2004
• Keywords: peoxidase / nitrotyrosine / heart / immunohistochemistry / myocardial infarction / homoglobin / myoglobin / myeloperoxidase

Research Abstract

We investigated whether some enzymes or proteins contribute to peroxidase-dependent tyrosine nitration are existed in the heart and what biochemical characteristics are contained in the peroxidases. Proteins contribute to tyrosine nitration are demonstrated in soluble fractions of rat's heart and showed a maximal tyrosine nitration capacity in pH 6.0 and were determined as hemoglobin and myoglobin. When cryosections of rat's heart were incubated in the presence of low concentrations of N02- and H202, immunohistochemical localization for nitrotyrosine was observed in a granular pattern in the myocytes. Moreover, we investigated the existence of peroxidase proteins contribute in tyrosine nitration in an ischemic heart or infracted lesions of the heart after isechemia reperfusion. Peroxidase-dependent tyrosine nitration capacity was observed in the coronary artery and determined as myeloperoxidase from neutrophils. However, immunostaining localization for nitrotyrosine was observed in infarcted lesions and not in the coronary artery of fixed heart sections or cryosections of the heart. Therefore, after removal of hemoglobin and myoblobin, although it is speculated that microperoxidases from the decomposition of cytochrome c may contribute to the tyrosine nitration of myocytes, it is not likely to consider it because of molecular weight of contributed proteins. In future, high molecular weight proteins should be investigated.

Research Products

• [Journal Article] Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice. (2006)
  • Author(s): Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
  • Journal Title: Life Sciences 78 Pages: 987-994
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice. (2006)
  • Author(s): Shibamori, M., Ogino, K., Kambayashi, Y., Ishiyama, H.
  • Journal Title: Life Sciences 78 Pages: 987-994
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Reactive nitrogen species formation in eosinophils and imbalance in nitric oxoxide metabolism are involved in atopic dermatitis-like skin lesions in NC/Nga mice. (2005)
  • Author(s): Kubo M, Kambayashi Y, Takemoto K, Okuda J, Muto M, Ogino K
  • Journal Title: Free Radic Res 39 Pages: 719-727
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Kinobeon A, purified from safflower's culture cells, is a novel and potent single oxygen quencher. (2005)
  • Author(s): Kambayashi Y, Takekoshi S, Nakano M, Shibamori M, Hitomi Y, Ogino K
  • Journal Title: Acta Bichemica Polonica 52 Pages: 903-907
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Reactive nitrogen species formation in eosinophils and imbalance in nitric oxide metabolism are involved in atopic dermatitis-like skin lesions in NC/Nga mice. (2005)
  • Author(s): Kubo, M., Kambayashi, Y., Takemoto, K., Okuda, J., Muto, M., Ogino K.
  • Journal Title: Free Radic.Res. 39 Pages: 719-727
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Kinobeon A, purified from safflower's culture cells, is a novel and potent singlet oxygen quencher. (2005)
  • Author(s): Kambayashi, Y., Takekoshi, S., Nakano, M., Shibamori, M., Hitomi, Y., Ogino, K.
  • Journal Title: Acta Bichemica Polonica 52 Pages: 903-907
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Induction of myeloperoxidase and nitrotyrosine formation in a human eosinophilic leukemia cell line, Eol-1. (2004)
  • Author(s): Kodama N, Kambaayashi Y, Kubo M, Yoneyama S, Nobukuni Y, Nakamura H, Ogino K
  • Journal Title: Biochem Funct 22 Pages: 105-112
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Induction of myeloperoxidase and nitrotyrosine formation in a human eosinophilic leukemia cell line, EoL-1. (2004)
  • Author(s): Kodama, N., Kambayashi, Y., Kubo, M., Yoneyama, S., Nobukuni, Y., Nakamura, H., Ogino, K.
  • Journal Title: Biochem.Funct. 22 Pages: 105-112
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis. (2004)
  • Author(s): Nakamura, H., Higashikawa, F., Miyagawa, K., Nobukuni, Y., Endo, T., Imai, T., Ozasa, K., Motohashi, Y., Matsuzaki, I., Sasahara, S., Hatta, K., Ogino, k., Eboshida, A.
  • Journal Title: Int.Arch.Allergy Immunol. 135 Pages: 40-43
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Reestimation of Cypridina luciferin analogues (MCLA) as a chemiluminescence probe to detect active oxygen species-cationary note for use of MCLA (2003)
  • Author(s): Kambayashi Y, Ogino K
  • Journal Title: J Toxicol Sci 28 Pages: 139-148
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High contribution contrast between the genes of eosinophil peroxidase and IL-4 receptor alpha-chain in Japanese cedar pollinosis. (2003)
  • Author(s): Nakamura H, Miyagawa K, Ogino K, Endo T, Imai T, Ozasa K, Motohashi Y, Matsuzaki I, Sasahara S, Hatta K, Eboshida A
  • Journal Title: J Allergy Clin Immun 112 Pages: 1127-1131
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Reestimation of Cypridina luciferin analogs (MCLA) as a chemiluminescence probe to detect active oxygen species -cautionary note for use of MCLA. (2003)
  • Author(s): Kambayashi, Y., Ogino, K.
  • Journal Title: J.Toxicol.Sci. 28 Pages: 139-148
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High contribution contrast between the genes of eosinophil peroxidase and IL-4 receptor alpha-chain in Japanese cedar pollinosis. (2003)
  • Author(s): Nakamura, H., Miyagawa, K., Ogino, K., Endo, T., Imai, T., Ozasa, K., Motohashi, Y., Matsuzaki, I., Sasahara, S., Hatta, K., Eboshida, A.
  • Journal Title: J.Allergy Clin.Immun. 112 Pages: 1127-1131
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Formation of superoxide anion during ferrous ion-catalyzed decomposition of linoleic acid hydroperoxide under aerobic condition. (2003)
  • Author(s): Kambayashi, Y., Tero-Kubota, S., Yamamoto, Y., Kato, M., Nakano, M., Yagi, K., Ogino, K.
  • Journal Title: J.Biochem. 134 Pages: 903-909
  • Description: 「研究成果報告書概要(欧文)」より