2003 Fiscal Year Annual Research Report

新規マウスWAPモチーフ蛋白ELM1,ELM2の機能解析とヒト相同遺伝子の単離

Research Project

Project/Area Number	14370194
Research Institution	Saitama Medical School
Principal Investigator	萩原弘一埼玉医科大学, 医学部, 教授 (00240705)
Co-Investigator(Kenkyū-buntansha)	永田真埼玉医科大学, 医学部, 助教授 (20211443)
Keywords	SWAM1 / SWAM2 / WAP / SLPI / elafin / 抗菌蛋白 / 自然免疫 / 染色体20番
Research Abstract	抗菌蛋白は,自然免疫の重要な構成要素の一つである.ElafinとSLPIは,抗菌活性とプロテアーゼ阻害活性の両方を有するWAPモチーフ蛋白で,それらの自然免疫における役割は,積極的に検討されている.我々は,マウスの新たなWAPモチーフ蛋白SWAM1,SWAM2をクローニングした.SWAM1とSWAM2は,シグナル配列と一つのWAPモチーフからなる蛋白で,elafinおよびSLPIと高い相同性を有していた.SWAM1は腎,上皮小体で定常的に発現しており,肺炎肺では発現が誘導された.SWAM2は舌で定常的に発現していた.SWAM1とSWAM2は大腸菌Staphylococcus aureusの増殖を10μMのIC90で阻害した.ヒトの相同遺伝子はLOC149709およびhuWAP2と考えられ,これらは他のWAP蛋白(WAP1,elafin, SLPI, HE4,eppin, C20orf170,LOC164237,WFDC3)と染色体20番上にクラスターをなして存在していた.これらの遺伝子は,共通の先祖遺伝子が重複して発生したと想像される.我々の研究は,WAPモチーフが抗菌蛋白形成の重要な骨格であることを示しており,他のWAPモチーフ蛋白も抗菌活性があるかどうか検索する必要があることを示している.

Research Products

(6 results)

All Other

All Publications (6 results)

[Publications] Hagiwara et al.: "Mouse SWAM1 and SWAM2 are antibacterial proteins composed of a single whey acidic protein (WAP) motif."Journal of Immunology. 170. 1973-1980 (2003)
[Publications] Nakamura et al.: "Increased susceptibility to LPS-induced endotoxin shock in secretory leukoprotease inhibitor (SLPI)-deficient mice"Journal of Experimental Medicine. 197. 669-674 (2003)
[Publications] Huqun et al.: "A naturally occurring p73 mutation in a p73-p53 double-mutant lung cancer cell line encodes p73a protein with a dominant negative function"Cancer Science. 94. 718-724 (2003)
[Publications] Fukushima et al.: "BRAF mutations in papillary carcinoma of the thyroid"Oncogene. 22. 6455-6457 (2003)
[Publications] Pradono et al.: "Gene transfer of thromboxane A(2) synthase and prostaglandin I(2) synthase antithetically altered tumor angiogenesis and tumor growth"Cancer Research. 49. 745-755 (2002)
[Publications] Kikuchi et al.: "Clarithromycin suppressed lipopolysaccharide-induced interleukin-8 production by human monocytes through AP-1 and NF-kB transcription factors."Journal of Antimicrobial Chemotherapy. 49. 745-755 (2002)

2003 Fiscal Year Annual Research Report

新規マウスWAPモチーフ蛋白ELM1,ELM2の機能解析とヒト相同遺伝子の単離

Principal Investigator

萩原 弘一 埼玉医科大学, 医学部, 教授 (00240705)

Research Products

[Publications] Hagiwara et al.: "Mouse SWAM1 and SWAM2 are antibacterial proteins composed of a single whey acidic protein (WAP) motif."Journal of Immunology. 170. 1973-1980 (2003)

[Publications] Nakamura et al.: "Increased susceptibility to LPS-induced endotoxin shock in secretory leukoprotease inhibitor (SLPI)-deficient mice"Journal of Experimental Medicine. 197. 669-674 (2003)

[Publications] Huqun et al.: "A naturally occurring p73 mutation in a p73-p53 double-mutant lung cancer cell line encodes p73a protein with a dominant negative function"Cancer Science. 94. 718-724 (2003)

[Publications] Fukushima et al.: "BRAF mutations in papillary carcinoma of the thyroid"Oncogene. 22. 6455-6457 (2003)

[Publications] Pradono et al.: "Gene transfer of thromboxane A(2) synthase and prostaglandin I(2) synthase antithetically altered tumor angiogenesis and tumor growth"Cancer Research. 49. 745-755 (2002)

[Publications] Kikuchi et al.: "Clarithromycin suppressed lipopolysaccharide-induced interleukin-8 production by human monocytes through AP-1 and NF-kB transcription factors."Journal of Antimicrobial Chemotherapy. 49. 745-755 (2002)

萩原弘一埼玉医科大学, 医学部, 教授 (00240705)