Development of improving method of respiratory function in newborn diaphragma hernia

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 14370402
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Yamagata University
• Principal Investigator: INUI Kiyoshige Yamagata Univ., Med., Assistant Proefssor, 医学部, 講師 (70250941)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Nobumasa Yamagata Unversity, Med., Assistant Professor, 医学部, 助手 (10333957)
• Project Period (FY): 2002 – 2003
• Keywords: chromatin / transdiggerentiation / acetyltransferase

Research Abstract

We speculated that there is a stem cell of myocardial cell in heart tissue. But in spite of our search for stem cell, no evidence of the existence of stem cell was derived. We thought that the stem cell in the heart might be provided from outside of heart tissue, such as bone marrow. Otherwise, we thought that transdifferentiation mechanism might be the one of the good target for the research of myocardial regeneration. Method : Rat thymus was excised under general anesthesia. Thymus was minced and Tlyphocytes were selected and filtered using magnetic cell sorter for getting CD34 positive and CD31hi fraction. Histone acetyltransferase was administered to the culture of CD34 positive and CD31hi rich cells. The ability of Hoechst33342 excretion was measured by flowcytometry. The effect of TGF beta and EGE for transdifferentiation was also studied. Pururipotent cells in the tissue or bone marrow may be differentiate by the transdifferentiation mechanism.