2004 Fiscal Year Final Research Report Summary
Stress responses and leukocyte functions in sepsis
| Project/Area Number |
14370488
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka University |
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Principal Investigator |
TANAKA Hirosi Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (90252676)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIMOTO Hisashi Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (90127241)
SHIMAZU Takeshi Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (50196474)
OGURA Hiroshi Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (70301265)
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| Project Period (FY) |
2002 – 2004
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| Keywords | sepsis / multiple organ failure / leukocyte / NF-κB / glucocorticoid receptor / G-CSF / stress response / 炎症性サイトカイン |
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| Research Abstract |
The objective of our study is as follows. (1) To evaluate the balance between intranuclear NF-κB and GR in PMNLs from patients with sepsis or trauma. (2) To evaluate leukocyte functions after severe insult. (3) To investigate gene expression following stress responses.
(1) The expression of NF-κB and GR in PMNLs were significantly enhanced in SIRS patients. There was a strong correlation between, these two transcriptional factors. The PMNL oxidative activity was enhanced and plasma concentrations of IL-6, IL-8, IL- 10 and cortisol were also elevated in SIRS patients. The balance between expression of NF-κB and GR may control PMNL activation and inflammatory response following severe insults. (2) Severe trauma or sepsis enhanced expression of heat shock proteins (HSPs) in leukocytes during the acute phase. In leukocytes with enhanced expression of HSP, oxidative activity is increased and apoptosis is inhibited. The enhanced expression of HSPs may play a role in regulating leukocyte function in patients with sepsis. Activated leukocytes also enhance production of leukocyte microparticles with increased adhesion molecules in patients with sepsis. Activated leukocyte microparticles may play a role in the pathogenesis of endothelial activation and leukocyte-endothelium interaction in the presence of sepsis. (3) We found that Na/K ATPases may contribute to maintain the cellular environment of astrocytes subjected to hypoxia/reoxygenation. In the animal experiment, G-CSF administration attenuates zymosan-induced shock without suppression of reactive oxygen species. In addition, we found the enhanced expression of insulin-like growth factor in the group with G-CSF treatment by DNA micro-array method
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Research Products
(33 results)
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[Journal Article] The balance between expression of intranuclear NF-κB and glucocorticoid receptor in polymorphonuclear leukocytes in SIRS patients.2005
Author(s)
Nakamori Y, Ogura H, Koh T, Fujita K, Tanaka H, Sumi Y, Hosotsubo H, Yoshiya K, Irisawa T, Kuwagata Y, Shimazu T, Sugimoto H
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Journal Title
J Trauma 59
Pages: 308-315
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] New experimental model of crush injury of the hindlimbs in rats.2005
Author(s)
Akimau, P, Yoshiya, K, Hosotsubo, H, Takakuwa, T, Tanaka, H, Sugimoto, H
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cerebrospinal fluid concentrations of anti-inflammatory mediators in early-phase severe traumatic brain injury.2005
Author(s)
Shiozaki, T, Hayakata, T, Tasaki, O, Hosotsubo, H, Fujita, K, Mouri, T, Tajima, G, Kajino, K, Nakae, H, Tanaka, H, Shimazu, T, Sugimoto, H
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hepatocyte growth factorin polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.2004
Author(s)
Matsushima, A, Ogura, H, Koh, T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo, H, Kuwagata, Y, Tanaka, H, Shimazu, T, Sugimoto, H
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Journal Title
J Trauma 56
Pages: 259-264
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Enhanced production of endothelial microparticles with increased binding to leukocytes in patients with severe systemic inflammatory response syndrome.2004
Author(s)
Ogura, H, Tanaka, H, Koh, T, Fujita, K, Fujimi, S, Nakamori, Y, Hosotsubo, H, Kuwagata, Y, Shimazu, T, Sugimoto, H
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Journal Title
J Trauma 56
Pages: 823-831
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Early activation of y S T lymphocytes in patients with severe systemic inflammatory response syndrome.2004
Author(s)
Matsushima, A, Ogura, H, Fujita, K, Koh, Taichin, Tanaka, H, Sumi, Y, Yoshiya, K, Hosotsubo, H, Kuwagata, Y, Shimazu, T, Sugimoto, H
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Increased production of leukocyte microparticles with enhanced expression of adhesion molecules from activated polymorphonuclear leukocytes in severely injured patients.2003
Author(s)
Fujimi, S, Ogura, H, Tanaka, H, Koh, T, Hosotsubo, H, Nakamori, Y, Kuwagata, Y, Shimazu, T, Sugimoto, H
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Journal Title
J.Trauma 54
Pages: 114-120
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Enhanced expression of intranuclear NF-κB in primed polymorphonuclear leukocytes in SIRS patients.2003
Author(s)
Nakamori, Y, Koh, T, Ogura, H, Tanaka, H, Fujimi, S, Kasai, K, Hosotsubo, H, Shimazu, T, Sugimoto, H
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Journal Title
J.Trauma 54
Pages: 253-260
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Paradoxical cytoskeleton and microparticle formation changes in monocytes and polymorphonuclear leukocytes in severe systemic inflammatory response syndrome patients.2003
Author(s)
Itakura(Sumi), Y, Ogura, H, Tanaka, H, Koh, T, Fujita, K, Fujimi, S, Nakamori, Y, Shimazu, T, Sugimoto, H
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Journal Title
J Trauma 55
Pages: 1125-1132
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Induction of mRNAs and proteins for Na/K ATPase a1 and b1 subunits following hypoxia/ reoxygenation in astrocytes.2003
Author(s)
Kasai, K, Yamashita, T, Yamaguchi, A, Yoshiya, K, Kawakita, A, Tanaka, H, Sugimoto, H, Tohyama, M
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Journal Title
Mol.Brain Res. 110
Pages: 38-44
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mild hypothermia reduces expression of heat shock protein 60 in leukocytes from severely head-injured patients2003
Author(s)
Hashiguchi, N, Shiozaki, T, Ogura, H, Tanaka, H, Koh, T, Noborio, M, Kuwagata, Y, Shimazu, T, Sugimoto, H
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Journal Title
J.Trauma 55
Pages: 1054-1060
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Profile of gene expression in the subventricular zone after traumatic brain injury.2003
Author(s)
Yoshiya, K, Tanaka, H, Kasai, K, Irisawa, T, Shiozaki, T, Sugimoto, H
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Journal Title
J Neurotrauma 20
Pages: 1147-1162
Description
「研究成果報告書概要(欧文)」より
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