2004 Fiscal Year Final Research Report Summary
Pest-sequencing analysis of periodontal pathogen Porphyromonas gingivalis : expression of its proteins by oral environmental fectors.
| Project/Area Number |
14370585
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKAYAMA Koji Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (80150473)
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| Co-Investigator(Kenkyū-buntansha) |
OHARA Naoya Nagasaki University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (70223930)
NAITO Mariko Nagasaki University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (20244072)
SHOJI Mikio Nagasaki University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (10336175)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Oral Anaerobic Bacteria / Porphyromonas gingivalis / Stationary phase / Oxidative stress / Universal stress protein |
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| Research Abstract |
Porphyromonas gingivals, an obligate anaerobic bacterium, is implicated as a major pathogen in the development and progression of chronic periodontitis. Although expression of several virulence factors of the bacterium has been found to be affected by environmental stress such as entrance into the stationary growth phase and heat, there is relatively little information on the mechanisms that may operate in the bacterium in response to environmental stress. In this study, we investigated the new protein (UstA) that was initially identified following the two dimensional gel analysis. Expression of UstA was upregulated in stationary phase or by exposure to atmospheric oxygen. N-terminal sequencing and database analysis with the P.gingivalis genome sequence revealed that the UstA-encoding gene (ustA) was located upstream of a homologue of the usp gene encoding the universal stress protein on the chromosome. The ustA gene appeared to be transcribed in a monocistronic fashion as revealed by primer extension and Northern blot analysis. To elucidate the role of UstA in the bacterium, chromosomal mutants carrying a disruption of the ustA gene were constructed. The ustA mutant grew slower than the wild type parent strain in rich medium, resulting in a lower yield in stationary phase. Furthermore, in this mutant, the expression levels of the P.gingivalis homologues of superoxide dismutase, thiol peroxidase, and thioredoxin were markedly higher than those in the wild type especially in stationary phase. The ustA mutant was more resistant to diamide, a thiol-specific oxidant, than the wild type. In addition, the ustA mutation suppressed hypersensitivities of the oxyR mutant to diamide, metronidazole, and mitomycin C. These results suggest that UstA may play a significant role in oxidative stress responses in the bacterium.
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Research Products
(46 results)
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[Journal Article] Novel stationary-phase-upregulated protein of Porphyromonas gingivalis influences the production of superoxide dismutase, thiol perxidase and thioredoxin.2005
Author(s)
Kikuchi, Y., Ohara, N., Sato, K., Yoshimura, M., Yukitake, H., Sakai, E., Shoji, M., Naito, M., Nakayama, K.
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Journal Title
Microbiology 151
Pages: 841-853
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of a new membrane-associated protein which influences transport/maturation of gingipains and adhesins of Porphyromonas gingivalis.2005
Author(s)
Sato, K., Sakai, E., Veith, P.D., Shoji, M., Kikuchi, Y., Yukitake, H., Ohara, N., Naito, M., Okamoto, K., Reynolds, E.C., Nakayama, K.
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Journal Title
J.Biol.Chem. 280(10)
Pages: 8668-8677
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inhibitory effect of green tea catechins on cysteine proteinases in Porphyromonas gingivalis.2004
Author(s)
Okamoto, M., Sugimoto, A., Leung, K.-P., Nakayama, K., Kamaguchi, A., Maeda, N.
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Journal Title
Oral Microbiol.Immunol. 19
Pages: 118-120
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Infection-induced up-regulation of the costimulatory molecule 4-1BB in osteoblastic cells and its inhibitory effect on M-CSF/RANKL-induced in vitro osteoclastogenesis.2004
Author(s)
Saito, K., Ohara, N., Hotokezaka, H., Fukumoto, S., Yuasa, K., Naito, M., Fujiwara, T., Nakayama, K.
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Journal Title
J.Biol.Chem. 279(14)
Pages: 13555-13563
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The major structural components of two cell-surface filaments of Porphyromonas gingivalis are matured through lipoprotein precursors.2004
Author(s)
Shoji, M., Naito, M., Yukitake, H., Sato, K., Sakai, E., Ohara, N., Nakayama, K.
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Journal Title
Mol.Microbiol. 52(5)
Pages: 1513-1525
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Autolysis of Porphyromonas gingivalis is accompnied by an increase in several periodontal pathogenic factors in the supernatant.2004
Author(s)
Kamaguchi, A., Nakano, M., Shoji, M., Nakamura, R., Sagane, Y., Okamoto, M., Watanabe, T., Ohyama, T., Ohta, M., Nakayama, K.
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Journal Title
Microbiol.Immunol. 48(7)
Pages: 541-545
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Roles of Arg- and Lys-gingipains in coaggregation of Porphyromonas gingivalis : identification of its responsible molecules in translation products oi rgpA,kgp,and hagA.2004
Author(s)
Abe, N., Baba, A., Takii, R., Nakayama, K., Kamaguchi, A., Shibata, Y., Abiko, Y., Okamoto, K., Kadowaki, T., Yamamoto, K.
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Journal Title
Biol.Chem. 385
Pages: 1041-1047
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Porphyromonas gingivalis induces receptor activator of NF-kB ligand expression on osteoblasts through activator protein-1 pathway.2004
Author(s)
Okahashi, N., Inaba, H., Nakagawa, I., Yamamura, T., Kuboniwa, M., Nakayama, K., Hamada, S., Amano, A.
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Journal Title
Infect.Immun. 72
Pages: 1706-1714
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of inactivation of the Arg- and/or Lys-gingipain gene on selected virulence and physiological properties of Porphyromonas gingivalis.2003
Author(s)
Grenier, D., Roy, S., Chandad, F., Plamondon, P., Yoshioka, M., Nakayama, K., Mayrand, D.
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Journal Title
Infect.Immun. 71
Pages: 4742-4748
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of Porphyromonas gingivalis Vesicles on Coaggregation of Staphylococcus aureus to Oral Microorganisms.2003
Author(s)
Kamaguchi, A, Nakayama, K, Ichiyama, S, Nakamura, R, Watanabe, T, Ohta, M, Baba, H, Ohyama, T.
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Journal Title
Curr.Microbiol. 47
Pages: 485-491
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Interleukin-4 directly inhibits tumor necrosis factor-a-mediated osteoclast formation in mouse bone marrow macrophages.2003
Author(s)
Kitaura, H, Nagata, N, Fujimura, Y, Hotokezaka, H, Tatamiya, M, Nakao, Yoshida, N, Nakayama, K
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Journal Title
Immunol.Lett. 88(3)
Pages: 193-198
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Adhesins encoded by the gingipain genes of Porphyromonas gingivalis are responsible for coaggregation with Prevotella intermedia2003
Author(s)
Kamaguchi, A., Ohyama, T., Sakai, E., Nakamura, R., Watanabe, T., Baba, H., Nakayama, K.
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Journal Title
Microbiology 149
Pages: 1257-1264
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Dps from the obligate anaerobe Porphyromonas gingivalis : purification, gene cloning, gene expression and mutant studies.2003
Author(s)
Ueshima, J., Shoji, M., Ratnayake, D.B., Abe, K., Yoshida, S., Yamamoto, K., Nakayama, K.
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Journal Title
Infect.Immun. 71(3)
Pages: 1170-1178
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of IL-12 on TNF-α-mediated osteoclast formation in bone marrow cells : apoptosis mediated by Fas/Fas ligand interaction.2002
Author(s)
Kitaura, H., Nagata, N., Fujimura, Y., Hotokezaka, H., Yoshida, N., Nakayama, K.
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Journal Title
J.Immunol. 169(9)
Pages: 4732-4738
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] U0126 and PD98059,specific inhibitors of MEK, accelerate differentiation of RAW264.7 cells into osteoclast-like cells.2002
Author(s)
Hotokezaka, H., Sakai, E., Kanaoka, K., Saito, K., Matsuo, K., Kitaura, H., Yoshida, N., Nakayama, K.
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Journal Title
J.Biol.Chem. 277(49)
Pages: 47366-47372
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis : cell surface polysaccharide as an anchorage for gingipains.2002
Author(s)
Shoji, M., Ratnayake, D.B., Shi, Y., Kadowaki, T., Yamamoto, K., Yoshimura, F., Akamine, A., Curtis, M.A., Nakayama, K.
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Journal Title
Microbiology 148
Pages: 1183-1191
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The antibacterial surface of dental implants based on the photocatalytic bactericidal effect.
Author(s)
Suketa, N., Sawase, T., Kitaura, H., Naito, M., Baba, K., Nakayama, K., Wennerberg, A., Atsuta, M.
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Journal Title
Clinical Implant Dentistry and Related Research (In press)
Description
「研究成果報告書概要(欧文)」より
