2004 Fiscal Year Final Research Report Summary
Molecular dissection of organization and dynamics of membrane lipid domains
| Project/Area Number |
14370753
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | RIKEN |
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Principal Investigator |
KOBAYASHI Toshihide RIKEN, Sphingolipid functions lab., Head of the laboratory, スフィンゴ脂質機能研究チーム, チームリーダー (60162004)
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| Co-Investigator(Kenkyū-buntansha) |
SHOGOMORI Hidehiko RIKEN, Sphingolipid functions lab., Researcher, スフィンゴ脂質機能研究チーム, 研究員 (80391986)
ISHITSUKA Reiko RIKEN, Lipid biology lab., Researcher, 小林脂質生物学研究室, 研究員 (60342747)
MAKINO Asami RIKEN, Sphingolipid functions lab., Research associate, スフィンゴ脂質機能研究チーム, リサーチアソシエイト (20373368)
MURATE Motohide RIKEN, Sphingolipid functions lab., Researcher, スフィンゴ脂質機能研究チーム, 研究員 (30311369)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Sphingomyelin / Lipid raft / Cholesterol / Lipid probe / Membrane domain / Lipid-binding toxin / Endocytosis / Flip-flop |
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| Research Abstract |
Whereas the bilayer organization of biomembranes can be reconstituted in artificial liposomes with a simple lipid composition, biological membranes contain thousands of different lipid species, whose cellular distribution is stringently controlled. This complex distribution of lipids suggests that the targeting of lipids is highly regulated and that cells require a complex lipid supramolecular organization within their membranes. Our aim is to understand the organization, assembly and function of specific lipids and lipid domains. For this purpose, we developed several lipid-specific probes and a new inhibitor of Sphingolipid biosynthesis. These tools, in conjunction with biochemical, biophysical and cell biological approaches are now starting to provide valuable information to understand lipid supramolecular organization. Application of our probes uncovered several aspects of cellular lipid organization. Major subjects performed during these three years are :
1.Characterization of sphingomyelin-specific toxin, lysenin
2.Studies on organization and dynamics of sphingomyelin
3.Characterization of sulfamisterin (AB5366), a new inhibitor of serine palmitoyltransferase
4.Studies on distribution and transport of cholesterol-rich membrane domains
5.Cellular distribution of phosphatidylethanolamine in yeast
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Research Products
(15 results)
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[Journal Article] Cinnamycin (Ro09-0198) promotes cell binding and toxicity by inducing transbilayer lipid movement.2003
Author(s)
Makino A, Baba T, Fujimoto K, Iwamoto K, Yano Y, Terada N, Ohno S, Sato SB, Ohta A, Umeda M, Matsuzaki K, Kobayashi T.
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Journal Title
J.Biol.Chem 278
Pages: 3204-3209
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Oligomerization and pore formation of a sphingomyelin-specific toxin, lysenin.2003
Author(s)
Yamaji-Hasegawa A, Makino A, Baba T, Senoh Y, Kimura-Suda H, Sato B S, Terada N, Ohno S, Kiyokawa E, Umeda M, Kobayashi T.
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Journal Title
J.Biol.Chem 278
Pages: 22762-22770
Description
「研究成果報告書概要(欧文)」より
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