2003 Fiscal Year Final Research Report Summary
Cis-acting site on bipolar migration of the replication origin on the E.coil chromosome
| Project/Area Number |
14380331
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
NIKI Hironori National Institute of Genetics, Radioisotope Center, Associate Professor, 放射線・アイソトープセンター, 助教授 (70208122)
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| Co-Investigator(Kenkyū-buntansha) |
OGATA Yasuyuki National Institute of Genetics, Radioisotope Center, Assistant Professor, 放射線・アイソトープセンター, 助手 (90344449)
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| Project Period (FY) |
2002 – 2003
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| Keywords | bacteria / migration / nucleoids / partitioning / segregation |
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| Research Abstract |
During replication of the Escherichia coil chromosome, the replicated Ori domains migrate towards opposite cell poles, suggesting that a cis-acting site for bipolar migration is located in this region. To identify this cis-acting site, a series of mutants was constructed by splitting subchromosomes from the original chromosome. One mutant, containing a 720 kb subchromosome, was found to be defective in the bipolar positioning of oriC. The creation of deletion mutants allowed the identification of migS, a 25 bp sequence, as the cis-acting site for the bipolar positioning of oriC. When migS was located at the replication terminus, the chromosomal segment showed bipolar positioning. migS was able to rescue bipolar migration of plasmid DNA containing, a mutation in the SopABC partitioning system. Interestingly, multiple copies of the migS sequence on a plasmid in trans inhibited the bipolar positioning of oriC. Taken together, these findings indicate that migS plays a crucial role in the bipolar positioning of oriC. In addition, real-time analysis of the dynamic morphological changes of nucleoids in wild-type and migS mutants suggests that bipolar positioning of the replicated oriC contributes to nucleoid organization.
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Research Products
(2 results)
