2003 Fiscal Year Final Research Report Summary

Breeding of a super yeast whichi produced alcohol directly from cellulose

Research Project

Project/Area Number	14560076
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	応用微生物学・応用生物化学
Research Institution	Osaka Prefecture University
Principal Investigator	ARAI Motoo Osaka Prefecture University, Osaka Prefecture University, Agriculture & Biological Sciences, Professor (80081537)
Co-Investigator(Kenkyū-buntansha)	KAWAGUCHI Takashi Osaka Prefecture University, Agriculture & Biological Sciences, Associate Professor (70195056)
Project Period (FY)	2002 – 2003
Keywords	cellulase / glucosidase / gene / yeast / Aspergillus
Research Abstract	We tried breeding a yeast which produced alcohol directly from cellulose. Cellulase genes were from Aspergillus aculeatus. Expression vector having different nutrition requirement and different expression modes was constructed. Finally, we succced the double expression of 2 endo cellulases. However, the expression of exo cellulases and beta-glucosidase could not be detected. The expression of beta glucosidase was detected slightly when secretion signal was changed. To know the mechanism of expression control of cellulase genes, the mechanism of CREA was investigated.

(2 results)