2003 Fiscal Year Final Research Report Summary
Evaluation of physiological functions of antioxidants by using proteome analysis
| Project/Area Number |
14560104
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食品科学・栄養科学
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| Research Institution | University of Miyazaki |
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Principal Investigator |
NISHIYAMA Kazuo University of Miyazaki, Faculty of Agriculture, Department of Biochemistry and Applied Biosciences, Associate Professor, 農学部, 助教授 (40164610)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAKIBARA Yoichi University of Miyazaki, Faculty of Agriculture, Department of Biochemistry and Applied Biosciences, Associate Professor, 農学部, 助教授 (90295197)
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| Project Period (FY) |
2002 – 2003
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| Keywords | antioxidant / oncogene / Ha-ras / cancer cell selective growth inhibition / HL-60 / apoptosis regulating protein / sulfotransferase / antioxidant metabolism |
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| Research Abstract |
Cancer cell specific growth inhibition by antioxidants and NAD(P)H oxidase inhibitors.
First we investigated the growth-inhibitory effects of five sulfur-containing antioxidants on oncogene-transformed rat fibroblast cells, 3Y1-ras and its counterpart, 3Y1 cells. All five antioxidants tested showed 3Y1-ras selective growth inhibition but those antioxidants didn't show cytotoxicity at 1 mM. In contrast to antioxidants, NAD(P)H oxidase inhibitors showed growth inhibition and induced cell death in 3Y1-ras cells at μM level.
Induction of apoptosis by lipoic acid in HL-60 cells.
In another approach to investigate the effects of antioxidants on cellular functions, we tested the effect of lipoic acid(LA), a sulfur containing antioxidant, on human leukemia cell line, HL-60. LA induced apoptosis in HL-60 cells through caspase dependent pathway. However, DNA ladder formation, a typical marker of apoptosis, was found prior caspase 3 activation, suggesting the involvement of capase independent pathwa
… More
y in LA induced apoptosis. To demonstrate the involvement of caspase independent pathway, we used caspase inhibitor and analyzed various kinds of apoptosis regulating proteins. We found that DNA ladder formation by LA in the presence of caspse inhibitor. In addition, nuclear localization of apoptosis inducing factor(AIF)and inhibition of DNA ladder formation by a Ca^<2+> chelator were observed at the early stage of apoptosis. Thus, we showed that LA induced apoptosis of HL-60 cells through both caspase dependent and independent pathways.
Antioxidant metabolism by sulfation.
In mammals, sulfation is known to be a major pathway for the detoxification of xenobiotics as well as the biotransformation of endogenous compounds such as steroid and thyroid hormones, catecholamines. In these compounds steroids and catecholamines are relevant antioxidants in the body. We explored the sulfation of these antioxidative hormones with respect to substrate specificities, structure-function relationship of sulfotransferase and effect of divalent metal cations on the enzyme activity. Less
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Research Products
(25 results)
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[Journal Article] Retronasal aroma and orthonasal aroma of cereal Shochu using retronasal aroma simulator (RAS)2005
Author(s)
Sakaida, H., Watashi, N., Nakahara, N., Kai, T., Sakakibara, Y., Nishiyama, K., Suiko, M.
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Journal Title
Nippon Shokuhin Kagnku Kogaku Kaishi(in Japanese) 52(1)
Pages: 19-26
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Zebrafish tyrosylprotein sulfotransferase : molecular cloning, expression, and functional characterization.2004
Author(s)
Mishiro, E., Liu, M.-Y., Sakakibara, Y., Suiko, M., Liu, M.-C.
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Journal Title
Biochem.Cell Biol. 82(2)
Pages: 295-303
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Purification and characterization of laminaran hydrolases from Trichoderma viride2003
Author(s)
Nobe, R., Sakakibara, Y., Fukuda, N., Yoshida, N., Ogawa, K., Suiko, M
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Journal Title
Biochem.Biosci.Biotech. 67巻・6号
Pages: 1349-1357
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Sulfonation of environmental estrogens by zebrafish cytosolic sulfotransferases2003
Author(s)
Ohkimoto, K., Sugaharaa, T., Sakakibara, Y., Suiko, M., Liu, M.Y., Carterc, G., Liu, M.C.
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Journal Title
Biochem.Biophys.Res.Commun. 309巻・1号
Pages: 7-11
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Sulfation of hydroxychlorobiphenyls: Molecular cloning, expression, and functional characterization of zebrafish SULT1 sulfotransferases.2003
Author(s)
Sugahara, T., Liu, C.-C., Pai, T.G., Collodi, P., Suiko, M., Sakakibara, Y., Nishiyama, K., Liu, M.-C.
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Journal Title
Eur.J.Biochem. 270(11)
Pages: 2404-2411
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sulfonation of environmental estrogens by zebrafish cytosolic sulfotransferases.2003
Author(s)
Ohkimoto, K., Sugahara, T., Sakakibara, Y., Suiko, M., Liu, M.-Y., Carter, G., Liu, M.-C.
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Journal Title
Biochim.Biophys.Res.Commun. 309(1)
Pages: 7-11
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characteristic flavor of buckwheat Shochu and comparison of volatile compounds from variety cereal Shochu.2003
Author(s)
Sakaida, H., Nakahara, N., Watashi, N., Kai, T., Nakajima, Y, Sakakibara, Y., Nishiyama, K., Fukuda, N., Suiko, M.
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Journal Title
Nippon Shokuhin Kagaku Kogaku Kaishi(in Japanese) 50(12)
Pages: 555-562
Description
「研究成果報告書概要(欧文)」より
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