2003 Fiscal Year Final Research Report Summary

CpG oligodeoxynucleotides enhance the non-specific Immune responses on carp

Research Project

Project/Area Number	14560156
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	General fisheries
Research Institution	University of Miyazaki
Principal Investigator	SAKAI Masahiro University of Miyazaki, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20178536)
Project Period (FY)	2002 – 2003
Keywords	DNA / CpG / Carp / immune response / cytokine
Research Abstract	The synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG were evaluated for its ability to enhance the non-specific immune response in carp, Cyprinus carpio. Intraperitoneal injection of CpG-ODNs to fish daily, 3. days, resulted in enhanced responses of phagocytic and nitroblue tetrazolium (NBT) activities in kidney phagocytic cells. This activation of kidney cells was observed for at least 7 days post injection. The serum lysozyme activity also increased in the fish treated with CpG-ODNs. These results indicated that unmethylated CpG DNA enhance the non-specific immune response in carp.

(5 results)