2003 Fiscal Year Final Research Report Summary
Analysis of physiological function of a focal adhesion protein vinexin
| Project/Area Number |
14560287
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KIOKA Noriyuki KYOTO UNIVERSITY, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (90234179)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Kazumitsu KYOTO UNIVERSITY, Graduate School of Agriculture, Professor, 農学研究科, 教授 (10151789)
MATSUO Michinori KYOTO UNIVERSITY, Graduate School of Agriculture, Assistant Professor, 農学研究科, 助手 (00335308)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Cell Adhesion / Vinexin / Vinculin / Anchorage-dependence / foal adhesion protein |
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| Research Abstract |
Vinexin is a focal adhesion protein belonging to vinexin family (vinexin, CAP (c-cbl associated protein), ArgBP2 (Arg-binding protein 2). Proteins in this family share a sorbin homology (SoHo) domain at their N-terminal half. Functions of SoHo domain are not well known, although it is seemed to be involved in the regulation of actin cytoskeleton. Here we screened the SoHo-binding protein to clarify the function of SoHo domains and found that the fragment of alpha actin could bound to vinexin SoHo domain in two-hybrid system. The interaction of SoHo domain with alpha actin was confirmed by pull down assay and co-immunoprecipitation. Furthermore, the SoHo domain of vinexin was co-precipitated with filamentous actin. Interestingly, SoHo domains of other proteins were also associated. with fliamentous actin. Together, these findings suggest that SoHo domain functions as an actin binding domain.
To elucidate the function of vinexin in detail, we analyzed the vinexin knockout mice. Vinexin knockout mice were born, matured and bred as wild type mice. To examine the function in cultured cell, we isolated primary fibroblast from embryo. Unlike to the overexpression of vinexin, knockout of vinexin gene did not affect the activation of ERK or anchorage-dependence of ERK signaling. However, it affected the cell motility. Together these finding suggested that vinexin is involved in cell migration.
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Research Products
(12 results)
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[Publications] Suwa, A.Mitsushima, M., Ito, T., Akamatsu, M., Ueda, K., Amachi, T., Kioka, N.: "Vinexin beta Regulates the Anchorage Dependence of ERK2 Activation Stimulated by Epidermal Growth Factor."J Biol Chem. 277. 13053-13058 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Tanaka, A.R., Abe-Dohmae, S., Ohnishi, T., Aoki, R., Morinaga, G., Okuhira, K.I., Ikeda, Y., Kano, F., Matsuo, M., Kioka, N., Amachi, T., Murata, M., Yokoyama, S., Ueda, K.: "Effects of mutations of ABCA1 in the first extracellular domain on subcellular trafficking and ATP binding/hydrolysis."J Biol Chem. 278. 8815-8819 (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] Wakabayashi, M., Ito, T., Mitsushima, M., Aizawa, S., Ueda, K., Amachi, T., Kioka, N.: "Interaction of lp-dlg/KIAA0583, a membrane-associated guanylate kinase family protein, with vinexin and beta-catenin at sites of cell-cell contact"J Biol Chem. 278. 21709-21714 (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] Tanaka, A.R, Tanabe, K., Morita, M., Kurisu, M., Kasiwayama, Y., Matsuo, M., Kioka, N., Amachi, T., Imanaka, T., Ueda, K.: "ATP binding/hydrolysis by and phosphorylation of peroxisomal ATP-binding cassette proteins PMP70 (ABCD3) and adrenoleukodystrophy protein (ABCD1)."J Biol Chem. 277. 40142-40147 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Townson, S, Dobrzycka, K.M., Lee, A.V., Air, M., Deng, W., Kang, K., Jiang, S., Kioka, N., Michaelis, K., Oesterreich, S.: "SAFB2 - A new SAFB homolog and estrogen receptor corepressor."J Biol Chem. 278. 20059-20068 (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] Suwa, A., Mitsushima, M., Ito, T., Akamatsu, M., Ueda, K., Amachi, T., Kioka, N.: "Vinexin beta Regulates the Anchorage Dependence of IERK2 Activation Stimulated by Epidermal Growth Factor."J Biol Chem. 277. 13053-13058 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tanaka, A.R., Abe-Dohmae, S., Ohnishi, T., Aoki, R., Morinaga, G., Okuhira, K., Ikeda, Y., Kano, F., Matsuo, M., Kioka, N., Amachi, T., Murata, M., Yokoyama, S., Ueda, K.: "Effects of mutations of ABCA1 in the first extracellular domain on subcellular trafficking and ATP binding/hydrolysis."J Biol Chem. 278. 8815-8819 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Wakabayashi, M., Ito, T., Mitsushima, M., Aizawa, S., Ueda, K., Amachi, T., Kioka, N.: "Interaction of lp-dlg/KIAAO583, a membrane-associated guanylate kinase family protein, with vinexin and beta-catenin at sites of cell-cell contact."J Biol Chem. 278. 21709-21714 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tanaka, A.R., Tanabe, K., Morita, M., Kufisu, M., Kasiwayama, Y., Matsuo, M., Kioka, N., Amachi, T., Imanaka, T., Ueda, K.: "ATP binding/hydrolysis by and phosphorylation of peroxisomal ATP-binding cassette proteins PMP7O (ABCD3) and adrenoleukodystrophy protein (ABCD1)."J Biol Chem. 277. 40142-40147 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Townson, S., Dobrzycka, K.M., Lee, A.V., Afr, M., Deng, W., Kang, K., Jiang, S., Kioka, N., Michaelis, K., Oesterreich, S.: "SAFB2 -A new SAFB homolog and estrogen receptor corepressor."J Biol Chem. 278. 20059-20068 (2003)
Description
「研究成果報告書概要(欧文)」より
