2003 Fiscal Year Final Research Report Summary
Study of simian TT virus (s-TTV) infection and its pathogenesis to humans
| Project/Area Number |
14570209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
ABE Kenji National Institute of Infectious Diseases, Department of Pathology, Senior Researcher, 感染病理部, 主任研究官 (60130415)
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| Project Period (FY) |
2002 – 2003
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| Keywords | TT virus (TTV) / simian TTV (s-TTV) / molecular epidemiology / zoonosis |
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| Research Abstract |
Recently, we identified TTV isolates from nonhuman primates and named them simian TTV (s-TTV). To investigate the prevalence of s-TTV in humans, we examined sera from healthy individuals and patients with liver diseases in Japan for the presence of s-TTV DNA by PCR assay. s-TTV DNA was determined by nested PCR using s-TTV-specific primers designed from untranslated region of s-TTV genome. s-TTV DNA sequence was detected in 3 of 200 (1.5%) healthy adults but none of 48 infants without liver disease. On the other hand, s-TTV DNA was detected in 30 of 287 (10.5%) Japanese patients with liver disease. s-TTV coinfection with hepatitis B virus and hepatitis C virus were present in 16.7% and 30% of these patients, respectively, while 53.3% of patients were positive for s-TTV alone. Nucleotide sequence analyses in 20 patients confirmed that these PCR products were derived from s-TTV genome sequences and nearly 85% identical to those of s-TTV prototypes from chimpanzees. Phylogenetic analysis demonstrated that all s-TTV isolates from humans were distinguished clearly from the human TTV isolates. Furthermore, s-TTV in humans was classified into 2 different genotypes as well as simians. Our results indicate that generally 10.5% of Japanese patients with liver diseases were infected with s-TTV. The routes of s-TTV transmission from animal to human require clarification.
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Research Products
(22 results)
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[Publications] Xin Ding, Young Nyun Park, Teresa Casanovas Taltavull, Swan N.Thung, Xiaoming Jin, Yi Jin, Nguyen Sao Trung, Yoshihiro Edamoto, Kenji Abe: "Geographic characterization of hepatitis virus infections, genotyping of hepatitis B virus, and p53 mutation in hepatocellular carcinoma analyzed by in situ detection of viral genomes from carcinoma tissues : Comparison among six different countries."Journal of Infectious Diseases. 56. 12-18 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Mitsuhiko Moriyama, Masaaki Taira, Hiroshi Matsumura, Hiroshi Aoki, Morio Mikuni, Miki Kaneko, Atsuo Shioda, Kayo Iwaguchi, Shinobu Arai, Sagiri Ichijima, Hiroko Iwasaki, Naohide Tanaka, Kenji Abe, Yasuyuki Arakawa: "Genotype analysis, using PCR with type-specific primers, of hepatitis B virus isolates from patients coinfected with hepatitis delta virus genotype II from Miyako Island, Japan."Intervirology. 46. 114-120 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Makoto Hirano, Xin Ding, Tian-Cheng Li, Naokazu Takeda, Hiroki Kawabata, Nobuo Koizumi, Teruki Kadosaka, Ikuo Goto, Toshiyuki Masuzawa, Masaji Nakamura, Katsuya Taira, Toshiro Kuroki, Tsutomu Tanikawa, Tetsutaro Sata, Haruo Watanabe, Kenji Abe: "Evidence for widespread infection of hepatitis E virus among wild rats in Japan."Hepatology Research. 27. 1-5 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Xin Ding, Tian-Cheng Li, Shigeki Hayashi, Naohiko Masaki, Tran TT Huy, Makoto Hirano, Mari Yamaguchi, Mitsugu Usui, Naokazu Takeda, Kenji Abe: "Present state of hepatitis E virus epidemiology in Tokyo, Japan."Hepatology Research. 27. 169-173 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Mitsuhiko Moriyama, Morio Mikuni, Wu Longren, Zhao Zi-Yi, Wang Xueqing, Shuh Oshiro, Hiroshi Matsumura, Hiroshi Aoki, Sagiri Ichijima, Hiroko Iwasaki, Naohide Tanaka, Kenji Abe, Yasuyuki Arakawa: "Epidemiology of SEN virus infection among patients with hepatitis B and C in China."Hepatology Research. 27. 174-180 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Kenji Abe, Eriko Hayakawa, Andrei V.Sminov, Anna L.Rossina, Xin Ding, Tran Thien-Tuan Huy, Tetsutaro Sata, Vasily F.Uchaikin: "Molecular epidemiology of hepatitis B, C, D and E viruses among children in Moscow, Russia."Journal of Clinical Virology. 30. 57-61 (2004)
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「研究成果報告書概要(欧文)」より
