2003 Fiscal Year Final Research Report Summary
Antifungal susceptibility of new genotype of Candida albicans strains with group 1 intron
| Project/Area Number |
14570231
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
MIKAMI Yuzuru Chiba University, Res.Cent.for Pathogenic Fungi and Microbial Toxicoses, Professor, 真菌医学研究センター, 教授 (40092100)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Candid a albicans / Candida dubliniensis / genotyping / group 1 intron / drug susceptibility / clinical isolates |
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| Research Abstract |
The genetic diversity of recent clinical isolates of Candida albicans in Japan and Thailand was studied on the basis of amplified DNA band lengths determined with a specific PCR Primer. Our analyses of 401 clinical isolates of C.albicans showed that they could be classified into five genotypes A, B, C, D (C.dubliniensis) and a new genotype E. Most of the strains from Japan as well as Thailand were genotype A, and C was a minor group. The genotype E was characterized to have a group 1 intron-like sequence, which is longer that hitherto reported ones ad which has a nucleotide sequence length of 962-bp. Our analysis of the 962-bp sequence indicated that it is composed of an intron similar to that of C.dubliniensis of 621-bp with a 341-bp insertion. Analysis of the sequence of ITS region of the E showed that the sequence is identical with those of C.albicans. Throught the study, the possible horizontal transfer of the group 1 intron between C.dubliniensis and C.albicans E was suggested. A high degree of correlation between the presence of group l intron in genotype E and the susceptibility to flucytosine was observed. Five and two strains of C.dubliniensis from Japan and Thailand, respectively. They were not isolates from AIDS patients, and not resistant to azole-type antifungals such as fluconazole. Most of the resistant strains were found to belong to genotype A, B, o r, C, in Japan and Thailand. Detailed mechanisms of resistant of C.albicans (not C.dubliniensis) to azole and flucytosine ant ifungals were analyzed using micro-array method using home made array system in our laboratory.
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Research Products
(16 results)
