2004 Fiscal Year Final Research Report Summary
Analysis of the function of L^* protein of Theiler's virus (TV) by a lentiviral expression system
| Project/Area Number |
14570273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | KANAZAWA MEDICAL UNIVERSITY |
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Principal Investigator |
OHARA Yoshiro Kanazawa Medical University, Department of Microbiology, Professor, 医学部, 教授 (50203914)
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| Co-Investigator(Kenkyū-buntansha) |
ASAKURA Kunihiko Kanazawa Medical University, Department of Microbiology, Associate Professor, 医学部, 助教授 (50333159)
KONTANI Yasuhide Kanazawa Medical University, Department of Microbiology, Research Associate, 医学部, 助手 (30367461)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Theiler's virus / L^* protein / L^*-expressing macrophages / a mutant virus / virus growth / apoptosis |
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| Research Abstract |
DA subgroup strains of TV persist in the CNS of infected mice and induce demyelination. The mechanism(s) of virus persistence and demyelination remains unknown. DA subgroup strains synthesize a 17-kDa protein, called L^*, from an initiation site out-of-frame with the polyprotein. The previous study using a mutant virus, DAL^* 1 (in which the L^* AUG is substituted by an ACG), showed that L^* contributes to virus growth and has an anti-apoptotic effect in macrophages. Therefore, we established macrophage cell lines that continuatively express L^*, in order to confirm its role in TV growth and apoptosis, which may be important in TMEV pathogenesis. A "gain of function" experiment using L^*-expressing macrophages demonstrated that L^* protein of TMEV is essential for virus growth and has an anti-apoptotic effect in those macrophage cell lines.
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Research Products
(18 results)
