2003 Fiscal Year Final Research Report Summary
The antigen recognition mechanism by dual receptors of cytotoxic T lymphocytes
| Project/Area Number |
14570283
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
TANABE Masao Univ.Ryukyus, Graduate Sch.Med.Sci., Associate Professor, 大学院・医学研究科, 助教授 (30049077)
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| Project Period (FY) |
2002 – 2003
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| Keywords | cytotoxic T lymphocytes / bone marrow cells / X-chromosome linked gene product / dendritic cell / dual-recognition / immunological tolerance |
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| Research Abstract |
We studied the specificity of cytotoxic T lymphocytes(CTLs) which were induced in mixed lymphocyte cultures(MLC) in vitro. We used the unique culture system that bone marrow(BM) cells from responder mice as accessory cells were added to the cultures, which contained both responder lymph node cells and allogeneic stimulator spleen cells. In this report, two different stimulatory dendritic cell(DC) populations were prepared from spleen cells, such as DEC-205+ cell depleted(Mac-1+ cell enriched;MDC) or Mac-1+ cell depleted(DEC-205+ cell enriched;LDC) spleen cells.
In allo-MLC, the CTL induced by the stimulation of MDC had the dual specificity for both allo major histocompatibility antigen complex(MHC) and allo X-chromosome linked gene products(XLGP) of stimulator cells. On the other hand, the CTL induced by the stimulation of LDC had the specificity for allo MHC alone.
In polyclonal CTL induction by concanayalin A, autologous, not allogeneic, spleen cells were used as stimulator cells. The CTL induced by MDC-stimuli had the dual specificity, such as polyclonal allo-MHC and stimulator-self-XLGP. On the contrary, when LDC-stimuli was used, the CTL having the dual specificity were specifically suppressed.
In conclusion, we suggested that not only MHC but also XLGP had the regulatory function of immune response, such as CTL induction in periphery, and among two DC subpopulations MDC could activate the immune response, the other hand, LDC could suppress that.
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