2003 Fiscal Year Final Research Report Summary
Anti-neural antibodies induced by brain injury and analysis of their antigen
| Project/Area Number |
14570587
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
INUZUKA Takashi Gifu University, School of Medicine, Professor, 医学部, 教授 (50184734)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUYAMA Zenjiro Gifu University, School of Medicine, Lecturer, 医学部, 助手 (80313881)
HOZUMI Isao Gifu University, School of Medicine, Associcate Professor, 医学部, 助教授 (20242430)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Keywords | anti-neural antibodies / brain injury / autoimmunity / aging |
|---|
| Research Abstract |
Objects of our studies were detection and analysis of anti-neural antibodies induced by experimental brain injury, which may elucidate significance of the specific anti-neural antibodies in some neurological disease.
1.Spraque-Dawley female rats (6 month, 2 year age) were used, Bilateral stub wounds were made with a dissecting knife, Sera from rats were collected before and 4 weeks after operation. Sera were examined if they contained newly synthesized anti-neural antibodies by the Western blot using rat cerebrum, brainstem and liver as antigens.
2.Sera from 3 patients with cerebral infarction were collected at the day of onset and one month later, 3 pairs Non pair had that, None newly synthesized anti-neural antibody was detected in rat or human. Sensitivity of the system, limited amount of blood from rats are thought of the possibility to fail detection of antibody. Inflammatory insult to brain should be examined if it cause the anti-neural antibodies.
|
