2003 Fiscal Year Final Research Report Summary
Characterization of Intimal Smooth Muscle Precursor Cells in Circulating Human Peripheral Blood : Potential Origin of Intimal Smooth Muscle Cells in Vascular Lesions
| Project/Area Number |
14570679
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
SUGIYAMA Seigo Kumamoto University, School of Medicine, Instructor, 医学部附属病院, 助手 (90274711)
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| Project Period (FY) |
2002 – 2003
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| Keywords | atherosclerosis / blood cells / bone marrow / vascular smooth muscle cells / inflammation / precursor cells / surface antigen / human |
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| Research Abstract |
Background Smooth muscle cells (SMC) play an important role in human vascular diseases. Several lines of evidence demonstrate that circulating smooth muscle precursor cells (SMPC) derived from bone marrow contribute to intimal hyperplasia in animal models.
Methods and Results We obtained large spindle cells expressing smooth muscle a-actin (a-SMA), denoted here as "smooth muscle-like cells" (SMLC), from human peripheral blood mononuclear cells (PBMC). SMLC derived from human PBMC proliferated readily and had a pro-inflammatory phenotype during early culture. After maturation, SMLC could contract and express major SMC-markers. We discovered PBMC-expressing a-SMA in the circulating blood ; they exhibited a CD14(+)-CD105(+) phenotype. Sorted CD14-CD105 double-positive PBMC could differentiate into SMLC. The number of CD14-CD105-bearing PBMC increased significantly in patients with coronary artery disease (CAD) compared to patients without CAD.
Conclusion These results support the novel concept that SMPC exist in circulating human blood and could potentially contribute to the pathogenesis of vascular diseases.
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Research Products
(13 results)
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[Publications] Sugiyama, S., Kugiyama, K., Aikawa, M., Nakamura, S., Ogawa, H., Libby, P.: "Hypochlorous Acid, a Macrophage Product, Induces Endothelial Apoptosis and Tissue Factor Expression. -Involvement of Myeloperoxidase-Mediated Oxidant in Plaque Erosion and Thrombogenesis-"Arterioscler Thromb Vasc Biol.. 24. 1-6 (2004)
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「研究成果報告書概要(欧文)」より
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[Publications] Fukushima, H., Sugiyama, S., Kugiyama, K., Honda, O., Nakamura, S., Koide, S., Sakamoto, T., Yoshimura, M., Ogawa, H.: "Prognostic Value of Remnant-like Lipoprotein Particles Levels in Patients with Coronary Artery Disease and Type-2 Diabetes Mellitus."J Am Coll Cardiol. (in press). (2004)
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「研究成果報告書概要(欧文)」より
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[Publications] Honda, O., Sugiyama, S., Kugiyama, K., Fukushima, H., Nakamura, S., Koide, S., Kojima, S., Hirai, N., Kawano, H., Soejima, H., Sakamoto, T., Yoshimura, M., Ogawa, H.: "Echolucent carotid plaques predict future coronary events in patients with coronary artery disease."J Am Coll Cardiol. 43(7). 1177-1184 (2004)
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「研究成果報告書概要(欧文)」より
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[Publications] Suzuki, S., Sugiyama, S., Usuku, H., Hirai, N., Kaikita, K., Sakashita, N., Sakamoto, T., Yoshimura, M., Ogawa, H.: "Heart failure with silent coronary artery spasm exhibiting microscopic focal myocardial necrosis and amyloid-deposition."Intern Med. 43(3). 199-203 (2004)
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「研究成果報告書概要(欧文)」より
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[Publications] Koide, S., Kugiyama, K., Sugiyama, S., Nakamura, S., Fukushima, H., Honda, O., Yoshimura, M., Ogawa, H.: "Association of polymorphism in glutamate-cysteine ligase catalytic subunit gene with coronary vasomotor dysfunction and myocardial infarction."J Am Coll Cardiol. 41(4). 539-545 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakamura, S., Sugiyama, S., Fujioka, D., Kawabata, K., Ogawa, H., Kugiyama, K.: "Polymorphism in glutamate-cysteine ligase modifier subunit gene is associated with impairment of nitric oxide-mediated coronary vasomotor function."Circulation. 108(12). 1425-1427 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Aikawa, M., Sugiyama, S., Hill, C.C., Voglic, S.J., Rabkin, E., Fukumoto, Y., Schoen, F.J., Witztum, J.L., Libby, P.: "Lipid lowering reduces oxidative stress and endothelial cell activation in rabbit atheroma."Circulation. 106(11). 1390-1306 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakamura, S., Kugiyama, K., Sugiyama, S., Miyamoto, S., Koide, S., Fukushima, H., Honda, O., Yoshimura, M, Ogawa, H.: "Polymorphism in the 5'-flanking region of human glutamate-cysteine ligase modifier subunit gene is associated with myocardial infarction."Circulation. 105(25). 2968-2973 (2002)
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「研究成果報告書概要(欧文)」より
